N Zarkesh1, H Nowzari, J L Morrison, J Slots. 1. School of Dentistry, Department of Periodontology, University of Southern California, Los Angeles 90089-0641, USA.
Abstract
BACKGROUND: Periodontal pathogens are detrimental to periodontal healing in barrier membrane-assisted periodontal therapy. Tetracycline-coating of barrier membranes may reduce levels of infecting pathogens. This study evaluated the clinical and microbiological effects of tetracycline-coated expanded polytetrafluoroethylene (T-ePTFE) barrier membranes in the treatment of 2- to 3-wall intraosseous periodontal lesions around mandibular molars. METHODS: Eleven patients received non-coated barrier membranes (ePTFE) and 11 patients received T-ePTFE barrier membranes. Tetracycline coating was performed by placing ePTFE membranes first in a 5% tridodecylmethylammonium chloride solution and then in a basic 3% tetracycline solution. Microbiological examination included conventional culture and DNA probe analyses. Barrier membranes were removed 6 weeks after insertion. RESULTS: At baseline, the periodontal lesion depth averaged 8.0 mm in the ePTFE treated group and 7.4 mm in the T-ePTFE group. At 1 year post-treatment, the mean gain of probing attachment was 1.9 mm in the ePTFE group and 3.3 mm in the T-ePTFE group (P = 0.02). At 3 minutes after membrane placement, suspected periodontal pathogens were detected in several ePTFE membranes but only in one T-ePTFE membrane. At 6 weeks, all membranes showed periodontal pathogens, including Porphyromonas gingivalis, Fusobacterium species, Peptostreptococcus micros, Bacteroides forsythus, and motile rods. CONCLUSIONS: This study suggests that the use of tetracycline-coated ePTFE barrier membranes can result in additional gain of clinical periodontal attachment, most likely due to the antimicrobial properties of tetracycline during initial healing.
BACKGROUND: Periodontal pathogens are detrimental to periodontal healing in barrier membrane-assisted periodontal therapy. Tetracycline-coating of barrier membranes may reduce levels of infecting pathogens. This study evaluated the clinical and microbiological effects of tetracycline-coated expanded polytetrafluoroethylene (T-ePTFE) barrier membranes in the treatment of 2- to 3-wall intraosseous periodontal lesions around mandibular molars. METHODS: Eleven patients received non-coated barrier membranes (ePTFE) and 11 patients received T-ePTFE barrier membranes. Tetracycline coating was performed by placing ePTFE membranes first in a 5% tridodecylmethylammonium chloride solution and then in a basic 3% tetracycline solution. Microbiological examination included conventional culture and DNA probe analyses. Barrier membranes were removed 6 weeks after insertion. RESULTS: At baseline, the periodontal lesion depth averaged 8.0 mm in the ePTFE treated group and 7.4 mm in the T-ePTFE group. At 1 year post-treatment, the mean gain of probing attachment was 1.9 mm in the ePTFE group and 3.3 mm in the T-ePTFE group (P = 0.02). At 3 minutes after membrane placement, suspected periodontal pathogens were detected in several ePTFE membranes but only in one T-ePTFE membrane. At 6 weeks, all membranes showed periodontal pathogens, including Porphyromonas gingivalis, Fusobacterium species, Peptostreptococcus micros, Bacteroides forsythus, and motile rods. CONCLUSIONS: This study suggests that the use of tetracycline-coated ePTFE barrier membranes can result in additional gain of clinical periodontal attachment, most likely due to the antimicrobial properties of tetracycline during initial healing.
Authors: Behnoush Khorsand; Satheesh Elangovan; Liu Hong; Michael S D Kormann; Aliasger K Salem Journal: J Biomed Mater Res B Appl Biomater Date: 2018-11-22 Impact factor: 3.368