| Literature DB >> 10505617 |
M Weih1, A Bergk, N K Isaev, K Ruscher, D Megow, M Riepe, A Meisel, I V Victorov, U Dirnagl, U Dirnagi.
Abstract
Sublethal ischemia leads to increased tolerance against subsequent ischemia. We investigated whether tolerance could also be elicited by mild respiratory-chain inhibition (chemical hypoxia) in a rat neuronal-cell enriched culture system. 3-Nitropropionic acid (3-NPA) caused a concentration-dependent inhibition of succinate-dehydrogenase. Two hours preconditioning with 3-NPA 24-48 h before oxygen-glucose deprivation (OGD) reduced neuronal damage morphologically and reduced lactate deydrogenase (LDH) release up to 72% compared to sham-treated sister cultures without 3-NPA. In an attempt to elucidate transcriptional mechanisms, we found no rapid translocation of the hypoxia-sensitive transcription factors N F-KB or hypoxia-inducible factor-I (HIF-I) at 3-NPA concentrations sufficient to trigger tolerance against OGD. In accordance to previous in vivo and brain slice data, we conclude that 3-NPA chemically induces tolerance against oxygen-glucose deprivation in vitro. However, the underlying mechanisms remain elusive.Entities:
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Year: 1999 PMID： 10505617 DOI： 10.1016/s0304-3940(99)00594-7
Source DB: PubMed Journal: Neurosci Lett ISSN： 0304-3940 Impact factor: 3.046