Literature DB >> 10503241

Transient expression of the beta-glucuronidase gene in tissues of Arabidopsis thaliana by bombardment-mediated transformation.

M Seki1, A Iida, H Morikawa.   

Abstract

Particle bombardment has proved to be useful for the transformation of plants. We have previously reported successful transient expression of the beta-glucuronidase (GUS) gene in cultured plant cells and tissues and the stable transformation of various plants using a pneumatic particle gun. In this chapter, we describe transient expression of the GUS gene in Arabidopsis thaliana leaves and roots using the pneumatic particle gun.

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Year:  1999        PMID： 10503241     DOI： 10.1007/BF02788683

Source DB:  PubMed          Journal:  Mol Biotechnol        ISSN： 1073-6085            Impact factor:   2.695


  15 in total

1.  High-velocity microprojectiles for delivering nucleic acids into living cells. 1987.

Authors:  R M Klein; E D Wolf; R Wu; J C Sanford
Journal:  Biotechnology       Date:  1992

2.  Development of the particle inflow gun for DNA delivery to plant cells.

Authors:  J J Finer; P Vain; M W Jones; M D McMullen
Journal:  Plant Cell Rep       Date:  1992-07       Impact factor: 4.570

3.  Optimization of delivery of foreign DNA into higher-plant chloroplasts.

Authors:  G N Ye; H Daniell; J C Sanford
Journal:  Plant Mol Biol       Date:  1990-12       Impact factor: 4.076

4.  Photoregulation of a phytochrome gene promoter from oat transferred into rice by particle bombardment.

Authors:  W B Bruce; A H Christensen; T Klein; M Fromm; P H Quail
Journal:  Proc Natl Acad Sci U S A       Date:  1989-12       Impact factor: 11.205

5.  Nutrient requirements of suspension cultures of soybean root cells.

Authors:  O L Gamborg; R A Miller; K Ojima
Journal:  Exp Cell Res       Date:  1968-04       Impact factor: 3.905

6.  Transient gene expression in maize, rice, and wheat cells using an airgun apparatus.

Authors:  J H Oard; D F Paige; J A Simmonds; T M Gradziel
Journal:  Plant Physiol       Date:  1990-02       Impact factor: 8.340

7.  Stable Transformation of Soybean Callus by DNA-Coated Gold Particles.

Authors:  P Christou; D E McCabe; W F Swain
Journal:  Plant Physiol       Date:  1988-07       Impact factor: 8.340

8.  Evidence That More than 90% of beta-Glucuronidase-Expressing Cells after Particle Bombardment Directly Receive the Foreign Gene in their Nucleus.

Authors:  T Yamashita; A Iida; H Morikawa
Journal:  Plant Physiol       Date:  1991-10       Impact factor: 8.340

9.  The intron of Arabidopsis thaliana polyubiquitin genes is conserved in location and is a quantitative determinant of chimeric gene expression.

Authors:  S R Norris; S E Meyer; J Callis
Journal:  Plant Mol Biol       Date:  1993-03       Impact factor: 4.076

10.  Transient expression of beta-glucuronidase in Arabidopsis thaliana leaves and roots and Brassica napus stems using a pneumatic particle gun.

Authors:  M Seki; Y Komeda; A Iida; Y Yamada; H Morikawa
Journal:  Plant Mol Biol       Date:  1991-08       Impact factor: 4.076
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  3 in total

1.  Differences in the processing of DNA ends in Arabidopsis thaliana and tobacco: possible implications for genome evolution.

Authors:  Nadiya Orel; Holger Puchta
Journal:  Plant Mol Biol       Date:  2003-03       Impact factor: 4.076

2.  Directly transforming PCR-amplified DNA fragments into plant cells is a versatile system that facilitates the transient expression assay.

Authors:  Yuming Lu; Xi Chen; Yuxuan Wu; Yanping Wang; Yuqing He; Yan Wu
Journal:  PLoS One       Date:  2013-02-26       Impact factor: 3.240

3.  Suitable transfection methods for single particle tracing in plant suspension cells.

Authors:  Janett Göhring; Nick Fulcher; Kurt Schilcher; Andrea Barta; Jaroslaw Jacak
Journal:  Plant Methods       Date:  2014-05-31       Impact factor: 4.993
  3 in total

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