| Literature DB >> 10503077 |
Abstract
The phage displaying antibody fragments were subjected to three rounds of panning with hepatitis A virus (HAV) antigen in solid phase. The eluted phage was enriched nearly 100 fold, and the percentage of recombinant clones increased from 25% to 100% after three rounds of panning. The HAV antigen-specific monoclonal antibody (McAb) was screened by sandwich ELISA, and the specificity of these antibodies was further confirmed by competitive inhibition ELISA.Entities:
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Year: 1998 PMID： 10503077
Source DB: PubMed Journal: Chin J Biotechnol ISSN： 1042-749X