Literature DB >> 10489469

Crystal engineering: deletion mutagenesis of the 24 kDa fragment of the DNA gyrase B subunit from Staphylococcus aureus.

G E Dale1, D Kostrewa, B Gsell, M Stieger, A D'Arcy.   

Abstract

The 24 kDa fragment of DNA gyrase B from Staphylococcus aureus was expressed in Escherichia coli and purified for crystallization. Crystals of the wild-type protein grew in the presence of cyclothialidine but proved difficult to reproduce. In order to improve the crystallization, the flexible regions of the protein were deleted by mutagenesis. The mutant proteins were analyzed by differential scanning calorimetry and the most stable mutants produced crystals. It was possible to reproducibly grow single well defined crystals in the microbatch system which belonged to the space group C2 and diffracted isotropically to approximately 2 A resolution.

Entities:  

Mesh：

Substances：

Year:  1999        PMID： 10489469     DOI： 10.1107/s0907444999008227

Source DB:  PubMed          Journal:  Acta Crystallogr D Biol Crystallogr        ISSN： 0907-4449


  7 in total

1.  New surface contacts formed upon reductive lysine methylation: improving the probability of protein crystallization.

Authors:  Pawel Sledz; Heping Zheng; Krzysztof Murzyn; Maksymilian Chruszcz; Matthew D Zimmerman; Mahendra D Chordia; Andrzej Joachimiak; Wladek Minor
Journal:  Protein Sci       Date:  2010-07       Impact factor: 6.725

2.  Identification of an auxiliary druggable pocket in the DNA gyrase ATPase domain using fragment probes.

Authors:  Xiaojie Huang; Junsong Guo; Qi Liu; Qiong Gu; Jun Xu; Huihao Zhou
Journal:  Medchemcomm       Date:  2018-07-04       Impact factor: 3.597

3.  Synthesis, spectroscopic and molecular docking studies on new schiff bases, nucleosides and α-aminophosphonate derivatives as antibacterial agents.

Authors:  Saad H Alotaibi; Hamada H Amer
Journal:  Saudi J Biol Sci       Date:  2020-10-16       Impact factor: 4.219

4.  Systematic replacement of lysine with glutamine and alanine in Escherichia coli malate synthase G: effect on crystallization.

Authors:  David M Anstrom; Leslie Colip; Brian Moshofsky; Eric Hatcher; S James Remington
Journal:  Acta Crystallogr Sect F Struct Biol Cryst Commun       Date:  2005-11-24

5.  Nα-1, 3-Benzenedicarbonyl-Bis-(Amino Acid) and Dipeptide Candidates: Synthesis, Cytotoxic, Antimicrobial and Molecular Docking Investigation.

Authors:  Ahmed M Naglah; Gaber O Moustafa; Ahmed A Elhenawy; Marwa M Mounier; Heba El-Sayed; Mohamed A Al-Omar; Abdulrahman A Almehizia; Mashooq A Bhat
Journal:  Drug Des Devel Ther       Date:  2021-03-25       Impact factor: 4.162

6.  (NZ)CH...O contacts assist crystallization of a ParB-like nuclease.

Authors:  Neil Shaw; Chongyun Cheng; Wolfram Tempel; Jessie Chang; Joseph Ng; Xin-Yu Wang; Sarah Perrett; John Rose; Zihe Rao; Bi-Cheng Wang; Zhi-Jie Liu
Journal:  BMC Struct Biol       Date:  2007-07-07

7.  Protein crystallization: Eluding the bottleneck of X-ray crystallography.

Authors:  Joshua Holcomb; Nicholas Spellmon; Yingxue Zhang; Maysaa Doughan; Chunying Li; Zhe Yang
Journal:  AIMS Biophys       Date:  2017-09-26
  7 in total

北京卡尤迪生物科技股份有限公司 © 2022-2023.