Literature DB >> 10488770

Using RT-PCR and bDNA assays to measure non-clade B HIV-1 subtype RNA.

C Pasquier1, K Sandres, G Salama, J Puel, J Izopet.   

Abstract

The performance of the new version of RT-PCR assay (Amplicor HIV-1 Monitor v1.5) was assessed. The quantification of non-B subtype HIV-1 plasma RNA (30A, 1C, 1D, 3E, 2F, 3G) obtained using Monitor v1.5 was compared to the former version of this assay (Monitor v1.0) and to the Quantiplex v2.0 bDNA assay. The new primers used in Monitor v1.5 were similar to the former version in both specificity and sensitivity. The new primers corrected the detection and quantification defect observed previously for HIV-1 non-B subtypes and gave slightly higher RNA concentrations than those measured using the bDNA assay (+0.39 log copies/ml).

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Year:  1999        PMID： 10488770     DOI： 10.1016/s0166-0934(99)00057-9

Source DB:  PubMed          Journal:  J Virol Methods        ISSN： 0166-0934            Impact factor:   2.014


  5 in total

1.  Assessment of agreement between the AMPLICOR HIV-1 MONITOR test versions 1.0 and 1.5.

Authors:  Charles E Hill; Alicia M Green; Jessica Ingersoll; Kirk A Easley; Frederick S Nolte; Angela M Caliendo
Journal:  J Clin Microbiol       Date:  2004-01       Impact factor: 5.948

2.  Comparison of the sensitivities and viral load values of the AMPLICOR HIV-1 MONITOR version 1.0 and 1.5 tests.

Authors:  Angela M Caliendo; Jessica Ingersoll; Alicia M Green; Frederick S Nolte; Kirk A Easley
Journal:  J Clin Microbiol       Date:  2004-11       Impact factor: 5.948

3.  Impact of human immunodeficiency virus type 1 (HIV-1) genetic diversity on performance of four commercial viral load assays: LCx HIV RNA Quantitative, AMPLICOR HIV-1 MONITOR v1.5, VERSANT HIV-1 RNA 3.0, and NucliSens HIV-1 QT.

Authors:  Priscilla Swanson; Carmen de Mendoza; Yagnya Joshi; Alan Golden; Richard L Hodinka; Vincent Soriano; Sushil G Devare; John Hackett
Journal:  J Clin Microbiol       Date:  2005-08       Impact factor: 5.948

4.  Evaluation of the abbott LCx HIV-1 RNA quantitative, a new assay for quantitative determination of human immunodeficiency virus type 1 RNA.

Authors:  N Zanchetta; G Nardi; L Tocalli; L Drago; C Bossi; F R Pulvirenti; C Galli; M R Gismondo
Journal:  J Clin Microbiol       Date:  2000-10       Impact factor: 5.948

5.  Viral load levels measured at set-point have risen over the last decade of the HIV epidemic in the Netherlands.

Authors:  Luuk Gras; Suzanne Jurriaans; Margreet Bakker; Ard van Sighem; Daniela Bezemer; Christophe Fraser; Joep Lange; Jan M Prins; Ben Berkhout; Frank de Wolf
Journal:  PLoS One       Date:  2009-10-07       Impact factor: 3.240
  5 in total

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