B S Edwards1, F Kuckuck, L A Sklar. 1. Cancer Research and Treatment Center, Departments of Cytometry and Pathology, University of New Mexico Health Sciences Center, Albuquerque, New Mexico 87131, USA.
Abstract
BACKGROUND: The tools for high throughput flow cytometry have been limited in part because of the requirement that the samples must flow under pressure. We describe a simple system for sampling repetitively from an open vessel. METHODS: Under computer control, the sample is loaded into a sample loop in a reciprocating eight-way valve by the action of a syringe. When the valve position is switched, the plug of sample in the sample loop is transported to the flow cytometer by a pressure-driven fluid line. By coupling the plug-forming capability to a second multi-port valve, samples can be delivered sequentially from separate vessels. RESULTS: The valve is able to deliver samples at rates ranging up to about 9 samples per minute. Each plug of sample has uniform delivery characteristics with a reproducible coefficient of variation (CV). Even at the highest sampling rate, carryover between samples is limited. CONCLUSIONS: Plug-flow flow cytometry has the potential to automate the delivery of small samples from unpressurized sources at rates compatible with many screening and assay applications. Copyright 1999 Wiley-Liss, Inc.
BACKGROUND: The tools for high throughput flow cytometry have been limited in part because of the requirement that the samples must flow under pressure. We describe a simple system for sampling repetitively from an open vessel. METHODS: Under computer control, the sample is loaded into a sample loop in a reciprocating eight-way valve by the action of a syringe. When the valve position is switched, the plug of sample in the sample loop is transported to the flow cytometer by a pressure-driven fluid line. By coupling the plug-forming capability to a second multi-port valve, samples can be delivered sequentially from separate vessels. RESULTS: The valve is able to deliver samples at rates ranging up to about 9 samples per minute. Each plug of sample has uniform delivery characteristics with a reproducible coefficient of variation (CV). Even at the highest sampling rate, carryover between samples is limited. CONCLUSIONS: Plug-flow flow cytometry has the potential to automate the delivery of small samples from unpressurized sources at rates compatible with many screening and assay applications. Copyright 1999 Wiley-Liss, Inc.
Authors: Petr Hyka; Thomas Züllig; Claudia Ruth; Verena Looser; Christian Meier; Joachim Klein; Karel Melzoch; Hans-Peter Meyer; Anton Glieder; Karin Kovar Journal: Appl Environ Microbiol Date: 2010-05-14 Impact factor: 4.792
Authors: J Paul Robinson; Valery Patsekin; Cheryl Holdman; Kathy Ragheb; Jennifer Sturgis; Ray Fatig; Larisa V Avramova; Bartek Rajwa; V Jo Davisson; Nicole Lewis; Padma Narayanan; Nianyu Li; C W Qualls Journal: J Lab Autom Date: 2012-09-10