BACKGROUND: Food allergy is a common complaint among patients with a broad spectrum of abdominal and extra-abdominal symptoms that must be distinguished from other more common non-immunological food intolerances. AIMS: To investigate whether human intestinal hypersensitivity reactions are associated with detectable release of inflammatory mediators from activated cells, which may serve as a biological marker of true allergic reactions. PATIENTS/ METHODS: In eight patients with food allergy and seven healthy volunteers, a closed-segment perfusion technique was used to investigate the effects of jejunal food challenge on luminal release of tryptase, histamine, prostaglandin D(2), eosinophil cationic protein, peroxidase activity, and water flux. RESULTS: Intraluminal administration of food antigens induced a rapid increase in intestinal release of tryptase, histamine, prostaglandin D(2), and peroxidase activity (p<0.05 v basal period) but not eosinophil cationic protein. The increased release of these mediators was associated with a notable water secretory response. CONCLUSIONS: These results suggest that human intestinal hypersensitivity reactions are characterised by prompt activation of mast cells and other immune cells, with notable and immediate secretion of water and inflammatory mediators into the intestinal lumen. Analysis of the profile of markers released into the jejunum after food provocation may be useful for the objective diagnosis of food allergy.
BACKGROUND:Food allergy is a common complaint among patients with a broad spectrum of abdominal and extra-abdominal symptoms that must be distinguished from other more common non-immunological food intolerances. AIMS: To investigate whether human intestinal hypersensitivity reactions are associated with detectable release of inflammatory mediators from activated cells, which may serve as a biological marker of true allergic reactions. PATIENTS/ METHODS: In eight patients with food allergy and seven healthy volunteers, a closed-segment perfusion technique was used to investigate the effects of jejunal food challenge on luminal release of tryptase, histamine, prostaglandin D(2), eosinophil cationic protein, peroxidase activity, and water flux. RESULTS: Intraluminal administration of food antigens induced a rapid increase in intestinal release of tryptase, histamine, prostaglandin D(2), and peroxidase activity (p<0.05 v basal period) but not eosinophil cationic protein. The increased release of these mediators was associated with a notable water secretory response. CONCLUSIONS: These results suggest that human intestinal hypersensitivity reactions are characterised by prompt activation of mast cells and other immune cells, with notable and immediate secretion of water and inflammatory mediators into the intestinal lumen. Analysis of the profile of markers released into the jejunum after food provocation may be useful for the objective diagnosis of food allergy.
Authors: N N Jarjour; W J Calhoun; E A Kelly; G J Gleich; L B Schwartz; W W Busse Journal: Am J Respir Crit Care Med Date: 1997-05 Impact factor: 21.405
Authors: H H Jacobi; L K Poulsen; C M Reimert; P S Skov; A K Ulfgren; I Jones; L B Elfman; H J Malling; N Mygind Journal: Int Arch Allergy Immunol Date: 1998-05 Impact factor: 2.749
Authors: J Bousquet; P Chanez; J Y Lacoste; I Enander; P Venge; C Peterson; S Ahlstedt; F B Michel; P Godard Journal: J Allergy Clin Immunol Date: 1991-10 Impact factor: 10.793
Authors: Nicolas Cenac; Anne-Marie Coelho; Cathy Nguyen; Steven Compton; Patricia Andrade-Gordon; Wallace K MacNaughton; John L Wallace; Morley D Hollenberg; Nigel W Bunnett; Rafael Garcia-Villar; Lionel Bueno; Nathalie Vergnolle Journal: Am J Pathol Date: 2002-11 Impact factor: 4.307
Authors: Eric B Brandt; Richard T Strait; Dan Hershko; Quan Wang; Emily E Muntel; Troy A Scribner; Nives Zimmermann; Fred D Finkelman; Marc E Rothenberg Journal: J Clin Invest Date: 2003-12 Impact factor: 14.808
Authors: Odd Helge Gilja; Jan G Hatlebakk; Svein Odegaard; Arnold Berstad; Ivan Viola; Christopher Giertsen; Trygve Hausken; Hans Gregersen Journal: World J Gastroenterol Date: 2007-03-07 Impact factor: 5.742