| Literature DB >> 10477350 |
T Nishikawa1, M Haresaku, K Adachi, M Masuda, M Hayashi.
Abstract
We have developed an in vivo micronucleus (MN) test that uses rat skin as the target organ. Sample preparation involves cold-treating the epidermis with trypsin, peeling it off with a fine forceps, treating it in hypotonic solution, and staining it with acridine orange (A.O.). We evaluated the assay using mitomycin C (MMC) and methyl methanesulfonate (MMS) as model clastogens, applying them as single and repeat treatments. Both chemicals induced a significant, dose-dependent increase in MN frequency in basal cells. One treatment per day for 3 days was optimal for MN induction.Entities:
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Year: 1999 PMID： 10477350 DOI： 10.1016/s1383-5718(99)00084-4
Source DB: PubMed Journal: Mutat Res ISSN： 0027-5107 Impact factor: 2.433