PURPOSE: The objective of this study was to determine whether human conjunctival epithelium synthesizes TFF-peptides (formerly P-domain peptides, trefoil factors), a family of mucin-associated secretory peptides of the gastrointestinal tract. METHODS: Expression of TFF-peptides in human conjunctiva was monitored by reverse transcription-polymerase chain reaction analysis. Antisera specific for TFF-peptides were used for immunohistochemistry to determine the presence and distribution of TFF-peptides in human conjunctiva. RESULTS: mRNA expression of TFF1 and TFF3, but not TFF2, was detected in human conjunctiva. TFF1 and TFF3 but not TFF2 are stored in conjunctival goblet cells only as revealed by immunofluorescence. CONCLUSIONS: Goblet cells of the human conjunctiva synthesize TFF1 and TFF3. These peptides, together with the secretory ocular mucin MUC5AC, may contribute to the rheological properties of the tear film. They also may influence healing of corneal wounds due to their motogenic properties.
PURPOSE: The objective of this study was to determine whether human conjunctival epithelium synthesizes TFF-peptides (formerly P-domain peptides, trefoil factors), a family of mucin-associated secretory peptides of the gastrointestinal tract. METHODS: Expression of TFF-peptides in human conjunctiva was monitored by reverse transcription-polymerase chain reaction analysis. Antisera specific for TFF-peptides were used for immunohistochemistry to determine the presence and distribution of TFF-peptides in human conjunctiva. RESULTS: mRNA expression of TFF1 and TFF3, but not TFF2, was detected in human conjunctiva. TFF1 and TFF3 but not TFF2 are stored in conjunctival goblet cells only as revealed by immunofluorescence. CONCLUSIONS: Goblet cells of the human conjunctiva synthesize TFF1 and TFF3. These peptides, together with the secretory ocular mucin MUC5AC, may contribute to the rheological properties of the tear film. They also may influence healing of corneal wounds due to their motogenic properties.
Authors: Adnana N Paunel-Görgülü; Andreas G Franke; Friedrich P Paulsen; Nicole Dünker Journal: Histochem Cell Biol Date: 2011-04-22 Impact factor: 4.304
Authors: K Kobayashi; H Ogata; M Morikawa; S Iijima; N Harada; T Yoshida; W R Brown; N Inoue; Y Hamada; H Ishii; M Watanabe; T Hibi Journal: Gut Date: 2002-08 Impact factor: 23.059
Authors: Friedrich P Paulsen; Chee-Wai Woon; Deike Varoga; Anne Jansen; Fabian Garreis; Kristin Jäger; Marita Amm; Daniel K Podolsky; Philipp Steven; Nicholas P Barker; Saadettin Sel Journal: J Biol Chem Date: 2008-03-07 Impact factor: 5.157