Literature DB >> 10475256

Improved soft-agar colony assay in a fluid processing apparatus.

A D Forsman1, A R Herpich, S K Chapes.   

Abstract

The standard method for quantitating bone marrow precursor cells has been to count the number of colony-forming units that form in semisolid (0.3%) agar. Recently we adapted this assay for use in hardware, the Fluid Processing Apparatus, that is flown in standard payload lockers of the space shuttle. When mouse or rat macrophage colony-forming units were measured with this hardware in ground-based assays, we found significantly more colony growth than that seen in standard plate assays. The improved growth correlates with increased agar thickness but also appears to be due to properties inherent to the Fluid Processing Apparatus. This paper describes an improved method for determining bone marrow macrophage precursor numbers in semisolid agar.

Entities:  

Keywords:  Non-programmatic

Mesh：

Year:  1999        PMID： 10475256     DOI： 10.1007/s11626-999-0044-2

Source DB:  PubMed          Journal:  In Vitro Cell Dev Biol Anim        ISSN： 1071-2690            Impact factor:   2.416


  24 in total

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Authors:  D Metcalf
Journal:  Science       Date:  1991-10-25       Impact factor: 47.728

2.  Cell proliferation inhibition in reduced gravity.

Authors:  P J Moos; H K Fattaey; T C Johnson
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3.  Gravity in mammalian organ development: differentiation of cultured lung and pancreas rudiments during spaceflight.

Authors:  B S Spooner; P Hardman; A Paulsen
Journal:  J Exp Zool       Date:  1994-07-01

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Authors:  D Metcalf; A W Burgess
Journal:  J Cell Physiol       Date:  1982-06       Impact factor: 6.384

5.  Comparative effects in vivo of recombinant murine interleukin 3, natural murine colony-stimulating factor-1, and recombinant murine granulocyte-macrophage colony-stimulating factor on myelopoiesis in mice.

Authors:  H E Broxmeyer; D E Williams; S Cooper; R K Shadduck; S Gillis; A Waheed; D L Urdal; D C Bicknell
Journal:  J Clin Invest       Date:  1987-03       Impact factor: 14.808

6.  Abrogation of TNF-mediated cytotoxicity by space flight involves protein kinase C.

Authors:  K M Woods; S K Chapes
Journal:  Exp Cell Res       Date:  1994-03       Impact factor: 3.905

7.  Pre-metatarsal skeletal development in tissue culture at unit- and microgravity.

Authors:  B J Klement; B S Spooner
Journal:  J Exp Zool       Date:  1994-07-01

8.  Functional characterization of mouse granulocytes and macrophages produced in vitro from bone marrow progenitors stimulated with interleukin 3 (IL-3) or granulocyte-macrophage colony-stimulating factor (GM-CSF).

Authors:  J G Bender; K L Unverzagt; P B Maples; Y Mehrotra; J Mellon; D E Van Epps; C C Stewart
Journal:  Exp Hematol       Date:  1992-10       Impact factor: 3.084

9.  In vitro chick pre-cardiac explant tissue differentiation during spaceflight on SpaceHab-02.

Authors:  J S van Twest; A Paulsen; B S Spooner
Journal:  Acta Anat (Basel)       Date:  1995

10.  Over-expression of hsp70 confers tumorigenicity to mouse fibrosarcoma cells.

Authors:  M Jäättelä
Journal:  Int J Cancer       Date:  1995-03-03       Impact factor: 7.396
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  1 in total

1.  Understanding macrophage differentiation during space flight: The importance of ground-based experiments before space flight.

Authors:  Stephen K Chapes; M Teresa Ortega
Journal:  Recent Pat Space Technol       Date:  2013-06-01
  1 in total

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