LIAISON Ferritin--an automated chemiluminescent immunoassay for the determination of Ferritin.

An immunoassay utilizing chemiluminescence and paramagnetic particles has been developed for the new fully automated random access LIAISON immunoanalyzer. The LIAISON Ferritin assay is a two-site immunoluminometric one-step assay using two highly specific monoclonal antibodies. A specially designed unique Reagent Integral contains all specific reagents. The cooled reagent compartment guarantees an on-board stability of at least 4 weeks. The assay works with a 2-point calibrated master curve. To perform, the assay, 10 microliters sample is added to 300 microliters tracer and 20 microliters antibody-coated magnetic particles. After 10 min incubation the particles are separated, washed and the chemiluminescent signal is generated. The time to first result is only 15 min. The assay with a unique extended standard range up to 3,000 ng/ml shows no high dose hook effect up to 150,000 ng/ml (spiked sera). The cross-reactivity to human liver ferritin is 100%, to human spleen ferritin 66% and to human heart ferritin 8%. Precision (within-run < 3%; between-run < 5%), linearity, recovery and sensitivity (< 0.2 ng/ml) are excellent. The assay shows a very good correlation to LlA-mat Ferritin (r = 0.994). In summary the LIAISON Ferritin run on the new LIAISON immunoanalyzer and is a very rapid and accurate method rapidly providing reliable results.