Literature DB >> 10466635

A simple method for constructing E1- and E1/E4-deleted recombinant adenoviral vectors.

H Mizuguchi1, M A Kay.   

Abstract

We previously developed a two-plasmid in vitro ligation method that did not require a recombination step to produce new recombinant E1- or E1/E3-deleted adenoviral vectors. In this study, we have modified the system to improve the simplicity of vector construction and, in addition, to allow for production of an E1/E4-deleted vector.

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Year:  1999        PMID： 10466635     DOI： 10.1089/10430349950017374

Source DB:  PubMed          Journal:  Hum Gene Ther        ISSN： 1043-0342            Impact factor:   5.695


  51 in total

1.  Autoinhibitory regulation of p73 by Delta Np73 to modulate cell survival and death through a p73-specific target element within the Delta Np73 promoter.

Authors:  Takahito Nakagawa; Masato Takahashi; Toshinori Ozaki; Ken-ichi Watanabe Ki; Satoru Todo; Hiroyuki Mizuguchi; Takao Hayakawa; Akira Nakagawara
Journal:  Mol Cell Biol       Date:  2002-04       Impact factor: 4.272

2.  Reduction of natural adenovirus tropism to mouse liver by fiber-shaft exchange in combination with both CAR- and alphav integrin-binding ablation.

Authors:  Naoya Koizumi; Hiroyuki Mizuguchi; Fuminori Sakurai; Teruhide Yamaguchi; Yoshiteru Watanabe; Takao Hayakawa
Journal:  J Virol       Date:  2003-12       Impact factor: 5.103

3.  Efficient Gene Transduction of Dispersed Islet Cells in Culture Using Fiber-Modified Adenoviral Vectors.

Authors:  Hiroyuki Hanayama; Kazuo Ohashi; Rie Utoh; Hirofumi Shimizu; Kazuya Ise; Fuminori Sakurai; Hiroyuki Mizuguchi; Hiroyuki Tsuchiya; Teruo Okano; Mitsukazu Gotoh
Journal:  Cell Med       Date:  2015-08-26

4.  Cloning and Large-Scale Production of High-Capacity Adenoviral Vectors Based on the Human Adenovirus Type 5.

Authors:  Eric Ehrke-Schulz; Wenli Zhang; Maren Schiwon; Thorsten Bergmann; Manish Solanki; Jing Liu; Philip Boehme; Theo Leitner; Anja Ehrhardt
Journal:  J Vis Exp       Date:  2016-01-28       Impact factor: 1.355

5.  Efficient generation of functional hepatocytes from human embryonic stem cells and induced pluripotent stem cells by HNF4α transduction.

Authors:  Kazuo Takayama; Mitsuru Inamura; Kenji Kawabata; Kazufumi Katayama; Maiko Higuchi; Katsuhisa Tashiro; Aki Nonaka; Fuminori Sakurai; Takao Hayakawa; Miho Kusuda Furue; Hiroyuki Mizuguchi
Journal:  Mol Ther       Date:  2011-11-08       Impact factor: 11.454

6.  Versican proteolysis mediates myocardial regression during outflow tract development.

Authors:  Christine B Kern; Russell A Norris; Robert P Thompson; W Scott Argraves; Sarah E Fairey; Leticia Reyes; Stanley Hoffman; Roger R Markwald; Corey H Mjaatvedt
Journal:  Dev Dyn       Date:  2007-03       Impact factor: 3.780

7.  Tumor vascular targeted delivery of polymer-conjugated adenovirus vector for cancer gene therapy.

Authors:  Xinglei Yao; Yasuo Yoshioka; Tomohiro Morishige; Yusuke Eto; Shogo Narimatsu; Yasuaki Kawai; Hiroyuki Mizuguchi; Jian-Qing Gao; Yohei Mukai; Naoki Okada; Shinsaku Nakagawa
Journal:  Mol Ther       Date:  2011-06-14       Impact factor: 11.454

8.  Qualitative and quantitative analysis of adenovirus type 5 vector-induced memory CD8 T cells: not as bad as their reputation.

Authors:  Maria Abildgaard Steffensen; Peter Johannes Holst; Sanne Skovvang Steengaard; Benjamin Anderschou Holbech Jensen; Christina Bartholdy; Anette Stryhn; Jan Pravsgaard Christensen; Allan Randrup Thomsen
Journal:  J Virol       Date:  2013-03-27       Impact factor: 5.103

9.  Functional compartmentalization of the plasma membrane of neurons by a unique acyl chain composition of phospholipids.

Authors:  Hideaki Kuge; Kana Akahori; Ken-ichi Yagyu; Koichi Honke
Journal:  J Biol Chem       Date:  2014-08-05       Impact factor: 5.157

10.  A simplified in vitro ligation approach to clone an E1B55k-deleted double-targeted conditionally-replicative adenovirus.

Authors:  Yosef S Haviv
Journal:  Virol J       Date:  2009-02-07       Impact factor: 4.099
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