Literature DB >> 10461219

Continual remodeling of postsynaptic density and its regulation by synaptic activity.

S Okabe1, H D Kim, A Miwa, T Kuriu, H Okado.   

Abstract

A postsynaptic density (PSD) protein, PSD-95, was tagged with green fluorescent protein (GFP-PSD-95) and expressed in cultured hippocampal neurons using recombinant adenoviruses. GFP-PSD-95 was selectively localized to excitatory postsynaptic sites. Time-lapse fluorescence imaging of hippocampal neurons revealed that >20% of GFP-PSD-95 clusters turned over within 24 hours. The appearance rate of clusters was higher than the disappearance rate, and this difference accounted for the gradual increase of the cluster density observed in culture. Dynamics of PSD-95 clusters were also inhibited by blockers of excitatory synaptic transmission. Continual PSD turnover and its regulation by synaptic activity may be important in activity-dependent remodeling of neuronal connections.

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Year:  1999        PMID： 10461219     DOI： 10.1038/12175

Source DB:  PubMed          Journal:  Nat Neurosci        ISSN： 1097-6256            Impact factor:   24.884


  74 in total

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Authors:  G T Macleod; P A Dickens; M R Bennett
Journal:  J Neurosci       Date:  2001-04-01       Impact factor: 6.167

2.  Modular transport of postsynaptic density-95 clusters and association with stable spine precursors during early development of cortical neurons.

Authors:  O Prange; T H Murphy
Journal:  J Neurosci       Date:  2001-12-01       Impact factor: 6.167

3.  Subunit heterogeneity of cytoplasmic dynein: Differential expression of 14 kDa dynein light chains in rat hippocampus.

Authors:  J Z Chuang; T A Milner; C H Sung
Journal:  J Neurosci       Date:  2001-08-01       Impact factor: 6.167

4.  Rapid redistribution of the postsynaptic density protein PSD-Zip45 (Homer 1c) and its differential regulation by NMDA receptors and calcium channels.

Authors:  S Okabe; T Urushido; D Konno; H Okado; K Sobue
Journal:  J Neurosci       Date:  2001-12-15       Impact factor: 6.167

5.  Spine formation and correlated assembly of presynaptic and postsynaptic molecules.

Authors:  S Okabe; A Miwa; H Okado
Journal:  J Neurosci       Date:  2001-08-15       Impact factor: 6.167

6.  Synchronized formation and remodeling of postsynaptic densities: long-term visualization of hippocampal neurons expressing postsynaptic density proteins tagged with green fluorescent protein.

Authors:  Tatsuhiko Ebihara; Izumi Kawabata; Shinichi Usui; Kenji Sobue; Shigeo Okabe
Journal:  J Neurosci       Date:  2003-03-15       Impact factor: 6.167

7.  PSD-95 regulates synaptic transmission and plasticity in rat cerebral cortex.

Authors:  Jean-Claude Béïque; Rodrigo Andrade
Journal:  J Physiol       Date:  2003-02-01       Impact factor: 5.182

8.  p250GAP, a novel brain-enriched GTPase-activating protein for Rho family GTPases, is involved in the N-methyl-d-aspartate receptor signaling.

Authors:  Takanobu Nakazawa; Ayako M Watabe; Tohru Tezuka; Yutaka Yoshida; Kazumasa Yokoyama; Hisashi Umemori; Akihiro Inoue; Shigeo Okabe; Toshiya Manabe; Tadashi Yamamoto
Journal:  Mol Biol Cell       Date:  2003-04-04       Impact factor: 4.138

9.  Tuning FlaSh: redesign of the dynamics, voltage range, and color of the genetically encoded optical sensor of membrane potential.

Authors:  Giovanna Guerrero; Micah S Siegel; Botond Roska; Eli Loots; Ehud Y Isacoff
Journal:  Biophys J       Date:  2002-12       Impact factor: 4.033

10.  Principles of long-term dynamics of dendritic spines.

Authors:  Nobuaki Yasumatsu; Masanori Matsuzaki; Takashi Miyazaki; Jun Noguchi; Haruo Kasai
Journal:  J Neurosci       Date:  2008-12-10       Impact factor: 6.167
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