J Yang1, Z Yang, F Chen. 1. Department of Burns and Plastic Surgery, Bethune International Peace Hospital, Shijiazhuang.
Abstract
OBJECTIVE: This study examined the changes in myocardial calcium transport function and what roles it played in cardiac dysfunction after severe burn injury. METHODS: Contraction relaxation properties of the left ventricule (LV) were studied in isolated heart preparations of Wistar rat 3, 8, 24 hours after 30% TBSA full thickness burn. Sarcoplasmic reticulum (SR) calcium transport function was measured by millipore filtration technique. RESULTS: The maximal rates of LV pressure elevation (+ dp/ dtmax) and depression (-dp/dtmax) in burn group were significantly lower than that in control group (P < 0.01). The initial rate and capacity of calcium uptake by SR decreased markedly in burn group than in control group (P < 0.01). In addition, calcium dependent ATPase activity and coupling ratio of SR were also markedly depressed. CONCLUSION: SR calcium transport function depression was closely related to cardiac contractile dysfunction, and the former is one of the important mechanism for cardiac contractile dysfunction after severe burn injury.
OBJECTIVE: This study examined the changes in myocardial calcium transport function and what roles it played in cardiac dysfunction after severe burn injury. METHODS: Contraction relaxation properties of the left ventricule (LV) were studied in isolated heart preparations of Wistar rat 3, 8, 24 hours after 30% TBSA full thickness burn. Sarcoplasmic reticulum (SR) calcium transport function was measured by millipore filtration technique. RESULTS: The maximal rates of LV pressure elevation (+ dp/ dtmax) and depression (-dp/dtmax) in burn group were significantly lower than that in control group (P < 0.01). The initial rate and capacity of calcium uptake by SR decreased markedly in burn group than in control group (P < 0.01). In addition, calcium dependent ATPase activity and coupling ratio of SR were also markedly depressed. CONCLUSION: SR calcium transport function depression was closely related to cardiac contractile dysfunction, and the former is one of the important mechanism for cardiac contractile dysfunction after severe burn injury.
Authors: Marc G Jeschke; David L Chinkes; Celeste C Finnerty; Gabriela Kulp; Oscar E Suman; William B Norbury; Ludwik K Branski; Gerd G Gauglitz; Ronald P Mlcak; David N Herndon Journal: Ann Surg Date: 2008-09 Impact factor: 12.969
Authors: Marc G Jeschke; Ronald P Mlcak; Celeste C Finnerty; William B Norbury; Gerd G Gauglitz; Gabriela A Kulp; David N Herndon Journal: Crit Care Date: 2007 Impact factor: 9.097