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Literature DB >> 10451105

Separation of double-stranded and single-stranded DNA in polymer solutions: II. Separation, peak width and resolution.

Abstract

The electrophoretic separation of single-stranded and double-stranded DNA has been examined, using a matrix of linear poly-N,N-dimethylacrylamide (pDMA). The dependence of peak spacing, peak width and resolution on important parameters such as polymer concentration, polymer chain length and electric field strength, has been studied. This work complements our systematic study on electrophoretic mobility under different conditions (C. Heller, Electrophoresis 1999, 20, 1962-1977), and will help to further optimize and improve high performance DNA separation in capillary electrophoresis with entangled polymer solutions.

Entities: Chemical

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Year: 1999 PMID： 10451105 DOI： 10.1002/(SICI)1522-2683(19990701)20:10<1978::AID-ELPS1978>3.0.CO;2-G

Source DB: PubMed Journal: Electrophoresis ISSN： 0173-0835 Impact factor: 3.535

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Cited

6 in total

1. Divergent dispersion behavior of ssDNA fragments during microchip electrophoresis in pDMA and LPA entangled polymer networks.

Authors: Christopher P Fredlake; Daniel G Hert; Thomas P Niedringhaus; Jennifer S Lin; Annelise E Barron
Journal: Electrophoresis Date: 2012-05 Impact factor: 3.535

6. Free-solution electrophoretic separations of DNA-drag-tag conjugates on glass microchips with no polymer network and no loss of resolution at increased electric field strength.

Authors: Jennifer Coyne Albrecht; Matthew B Kerby; Thomas P Niedringhaus; Jennifer S Lin; Xiaoxiao Wang; Annelise E Barron
Journal: Electrophoresis Date: 2011-04-18 Impact factor: 3.535

6 in total

Separation of double-stranded and single-stranded DNA in polymer solutions: II. Separation, peak width and resolution.

1. Divergent dispersion behavior of ssDNA fragments during microchip electrophoresis in pDMA and LPA entangled polymer networks.

2. A 265-base DNA sequencing read by capillary electrophoresis with no separation matrix.

3. Identification of PCR products using PNA amphiphiles in micellar electrokinetic chromatography.

Review 4. Beyond gel electrophoresis: microfluidic separations, fluorescence burst analysis, and DNA stretching.

5. Analysis of inorganic polyphosphates by capillary gel electrophoresis.

6. Free-solution electrophoretic separations of DNA-drag-tag conjugates on glass microchips with no polymer network and no loss of resolution at increased electric field strength.