Literature DB >> 10450089

The free energies for mutating S27 and W79 to alanine in streptavidin and its biotin complex: the relative size of polar and nonpolar free energies on biotin binding.

R W Dixon1, P Kollman.   

Abstract

We have carried out calculations on the relative free energy of binding of biotin and its S27A and W79A mutants to streptavidin. Consistent with earlier suggestions by Miyamoto and Kollman from free energy component analysis and recent experiments by Stayton and coworkers, the reduction in binding strength by the W79A mutant is significantly larger than that of the S27A mutant. Proteins 1999;36:471-473. Copyright 1999 Wiley-Liss, Inc.

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Year:  1999        PMID： 10450089

Source DB:  PubMed          Journal:  Proteins        ISSN： 0887-3585


  6 in total

1.  Ser45 plays an important role in managing both the equilibrium and transition state energetics of the streptavidin-biotin system.

Authors:  D E Hyre; I Le Trong; S Freitag; R E Stenkamp; P S Stayton
Journal:  Protein Sci       Date:  2000-05       Impact factor: 6.725

2.  Context-dependent mutations predominate in an engineered high-affinity single chain antibody fragment.

Authors:  Katarina S Midelfort; K Dane Wittrup
Journal:  Protein Sci       Date:  2006-02       Impact factor: 6.725

3.  Extending Bell's model: how force transducer stiffness alters measured unbinding forces and kinetics of molecular complexes.

Authors:  Emily B Walton; Sunyoung Lee; Krystyn J Van Vliet
Journal:  Biophys J       Date:  2008-01-04       Impact factor: 4.033

4.  Cooperative hydrogen bond interactions in the streptavidin-biotin system.

Authors:  David E Hyre; Isolde Le Trong; Ethan A Merritt; John F Eccleston; N Michael Green; Ronald E Stenkamp; Patrick S Stayton
Journal:  Protein Sci       Date:  2006-02-01       Impact factor: 6.725

5.  Very empirical treatment of solvation and entropy: a force field derived from log Po/w.

Authors:  G E Kellogg; J C Burnett; D J Abraham
Journal:  J Comput Aided Mol Des       Date:  2001-04       Impact factor: 3.686

6.  The origins of femtomolar protein-ligand binding: hydrogen-bond cooperativity and desolvation energetics in the biotin-(strept)avidin binding site.

Authors:  Jason DeChancie; K N Houk
Journal:  J Am Chem Soc       Date:  2007-04-07       Impact factor: 15.419
  6 in total

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