OBJECTIVE: To evaluate the IgG immune response to HIV-1 in colostrum. METHODS: Paired serum and colostrum were collected from 16 asymptomatic HIV-1-infected women. IgG to gp160 and to four peptides (gp41 immunodominant DI domain, gp41/Id; EDLKWA epitope of DIII domain, gp41/K; gp120 C-terminus, gp120/Ct; V3 loop, gp120/V3) were evaluated in all samples. Functional activity of purified IgG was assessed for the ability to block transcytosis of cell-associated HIV-1 through a tight monolayer of endometrial epithelial cell line HEC1. RESULTS: IgG antibody to gp160 and to the four env-encoded synthetic peptides were detected in all specimens. The mean specific activity of IgG to gp41/K was 4.2 fold higher in colostrum than in paired serum. In contrast, mean specific activities of IgG to gp160 and gp41/Id were twofold higher in serum than in paired colostrum. Mean specific activities of IgG to gp120/V3 and to gp120/Ct were similar in systemic and milk compartments. Functional activity of IgG was evaluated in six paired serum and colostrum: in two women, serum IgG was 3.0 and 7.6 fold more efficient in blocking transcytosis than colostrum IgG; in one patient, colostrum IgG exhibited a 28 fold higher inhibitory capacity than serum IgG; in the remaining patients, serum and colostrum IgG demonstrated similar inhibitory activities against transcytosis of HIV. CONCLUSION: These features are consistent with a compartmentalization of the humoral IgG immune response to HIV within the mammary gland. Some HIV-1 antigens are able to induce a strong humoral mucosal immune response which may be of relevance for the design of a mucosal vaccine against HIV-1.
OBJECTIVE: To evaluate the IgG immune response to HIV-1 in colostrum. METHODS: Paired serum and colostrum were collected from 16 asymptomatic HIV-1-infectedwomen. IgG to gp160 and to four peptides (gp41 immunodominant DI domain, gp41/Id; EDLKWA epitope of DIII domain, gp41/K; gp120 C-terminus, gp120/Ct; V3 loop, gp120/V3) were evaluated in all samples. Functional activity of purified IgG was assessed for the ability to block transcytosis of cell-associated HIV-1 through a tight monolayer of endometrial epithelial cell line HEC1. RESULTS: IgG antibody to gp160 and to the four env-encoded synthetic peptides were detected in all specimens. The mean specific activity of IgG to gp41/K was 4.2 fold higher in colostrum than in paired serum. In contrast, mean specific activities of IgG to gp160 and gp41/Id were twofold higher in serum than in paired colostrum. Mean specific activities of IgG to gp120/V3 and to gp120/Ct were similar in systemic and milk compartments. Functional activity of IgG was evaluated in six paired serum and colostrum: in two women, serum IgG was 3.0 and 7.6 fold more efficient in blocking transcytosis than colostrum IgG; in one patient, colostrum IgG exhibited a 28 fold higher inhibitory capacity than serum IgG; in the remaining patients, serum and colostrum IgG demonstrated similar inhibitory activities against transcytosis of HIV. CONCLUSION: These features are consistent with a compartmentalization of the humoral IgG immune response to HIV within the mammary gland. Some HIV-1 antigens are able to induce a strong humoral mucosal immune response which may be of relevance for the design of a mucosal vaccine against HIV-1.
Authors: Genevieve G Fouda; Nicole L Yates; Justin Pollara; Xiaoying Shen; Glenn R Overman; Tatenda Mahlokozera; Andrew B Wilks; Helen H Kang; Jesus F Salazar-Gonzalez; Maria G Salazar; Linda Kalilani; Steve R Meshnick; Beatrice H Hahn; George M Shaw; Rachel V Lovingood; Thomas N Denny; Barton Haynes; Norman L Letvin; Guido Ferrari; David C Montefiori; Georgia D Tomaras; Sallie R Permar Journal: J Virol Date: 2011-07-06 Impact factor: 5.103
Authors: Laura Heath; Susan Conway; Laura Jones; Katherine Semrau; Kyle Nakamura; Jan Walter; W Don Decker; Jason Hong; Thomas Chen; Marintha Heil; Moses Sinkala; Chipepo Kankasa; Donald M Thea; Louise Kuhn; James I Mullins; Grace M Aldrovandi Journal: PLoS One Date: 2010-04-20 Impact factor: 3.240
Authors: Steffanie Sabbaj; Bradley H Edwards; Mrinal K Ghosh; Katherine Semrau; Sanford Cheelo; Donald M Thea; Louise Kuhn; G Douglas Ritter; Mark J Mulligan; Paul A Goepfert; Grace M Aldrovandi Journal: J Virol Date: 2002-08 Impact factor: 5.103