Literature DB >> 10448953

Improved high-performance liquid chromatographic assay for the determination of "high-energy" phosphates in mammalian skeletal muscle. Application to a single-fibre study in man.

C Karatzaferi1, A De Haan, C Offringa, A J Sargeant.   

Abstract

A sensitive and reproducible method for the determination of adenine nucleotides (ATP, IMP) and creatine compounds [creatine (Cr), phosphocreatine (PCr)] in freeze-dried single human muscle fibre fragments is presented. The method uses isocratic reversed-phase high-performance liquid chromatography of methanol extracts. Average retention times (min) of ATP, IMP and PCr, Cr from standard solutions were 10.6+/-0.42, 2.11+/-0.06 (n=6) and 10.5+/-0.31 and 1.19+/-0.02 (n=9), respectively. Detection limits in extracts from muscle fibre fragments were 2.0, 1.0, 3.0 and 2.0 mmol/kg dm, respectively. The assay was found successful for analysis of fibre-fragments weighing > or = 1 microg.

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Year:  1999        PMID： 10448953     DOI： 10.1016/s0378-4347(99)00221-2

Source DB:  PubMed          Journal:  J Chromatogr B Biomed Sci Appl        ISSN： 1387-2273


  4 in total

1.  Post-tetanic potentiation increases energy cost to a higher extent than work in rat fast skeletal muscle.

Authors:  F Abbate; J Van Der Velden; G J Stienen; A De Haan
Journal:  J Muscle Res Cell Motil       Date:  2001       Impact factor: 2.698

2.  Metabolically assessed muscle fibre recruitment in brief isometric contractions at different intensities.

Authors:  J G M Beltman; A de Haan; H Haan; H L Gerrits; W van Mechelen; A J Sargeant
Journal:  Eur J Appl Physiol       Date:  2004-05-08       Impact factor: 3.078

3.  Phosphorylated guanidinoacetate partly compensates for the lack of phosphocreatine in skeletal muscle of mice lacking guanidinoacetate methyltransferase.

Authors:  Hermien E Kan; W Klaas Jan Renema; Dirk Isbrandt; Arend Heerschap
Journal:  J Physiol       Date:  2004-07-29       Impact factor: 5.182

4.  Transient alterations of creatine, creatine phosphate, N-acetylaspartate and high-energy phosphates after mild traumatic brain injury in the rat.

Authors:  Stefano Signoretti; Valentina Di Pietro; Roberto Vagnozzi; Giuseppe Lazzarino; Angela M Amorini; Antonio Belli; Serafina D'Urso; Barbara Tavazzi
Journal:  Mol Cell Biochem       Date:  2009-08-18       Impact factor: 3.396
  4 in total

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