OBJECTIVE: To look for oligosaccharide structural variants of IgG that may be unique to specific rheumatic diseases. METHODS: Using normal-phase high-performance liquid chromatography technology, a comparison was made of the oligosaccharide pools released from serum IgG from patients with systemic lupus erythematosus (SLE) (n = 10), ankylosing spondylitis (AS) (n = 10), primary Sjögren's syndrome (n = 6), juvenile chronic arthritis (JCA) (n = 13), psoriatic arthritis (n = 9), rheumatoid arthritis (RA) (n = 5), and healthy control individuals (n = 19). RESULTS: The oligosaccharide pools were resolved into 13 peaks and the relative proportions of the peaks in each disease group was significantly different from that in healthy controls (P < 0.0001-0.05). A characteristic serum IgG oligosaccharide profile, or sugar print, for each of the rheumatic diseases was found. The sugar prints exhibited a range of glycosylation patterns whereby all RA (P < 0.0001) and JCA (P < 0.006) patients had predominantly agalactosyl structures, while SLE (P < 0.03-0.0001) and AS (P < 0.025-0.0001) patients had predominantly digalactosyl structures. CONCLUSION: The data suggest that each disease is associated with a specific mechanism that gives rise to alterations in the normal glycosylation pattern of IgG. Sugar printing of IgG is therefore a potential means for the differentiation of rheumatic diseases and may provide insight into disease pathogenesis.
OBJECTIVE: To look for oligosaccharide structural variants of IgG that may be unique to specific rheumatic diseases. METHODS: Using normal-phase high-performance liquid chromatography technology, a comparison was made of the oligosaccharide pools released from serum IgG from patients with systemic lupus erythematosus (SLE) (n = 10), ankylosing spondylitis (AS) (n = 10), primary Sjögren's syndrome (n = 6), juvenile chronic arthritis (JCA) (n = 13), psoriatic arthritis (n = 9), rheumatoid arthritis (RA) (n = 5), and healthy control individuals (n = 19). RESULTS: The oligosaccharide pools were resolved into 13 peaks and the relative proportions of the peaks in each disease group was significantly different from that in healthy controls (P < 0.0001-0.05). A characteristic serum IgG oligosaccharide profile, or sugar print, for each of the rheumatic diseases was found. The sugar prints exhibited a range of glycosylation patterns whereby all RA (P < 0.0001) and JCA (P < 0.006) patients had predominantly agalactosyl structures, while SLE (P < 0.03-0.0001) and AS (P < 0.025-0.0001) patients had predominantly digalactosyl structures. CONCLUSION: The data suggest that each disease is associated with a specific mechanism that gives rise to alterations in the normal glycosylation pattern of IgG. Sugar printing of IgG is therefore a potential means for the differentiation of rheumatic diseases and may provide insight into disease pathogenesis.
Authors: Altan Ercan; Jing Cui; Dereck E W Chatterton; Kevin D Deane; Melissa M Hazen; William Brintnell; Colin I O'Donnell; Lezlie A Derber; Michael E Weinblatt; Nancy A Shadick; David A Bell; Ewa Cairns; Daniel H Solomon; V Michael Holers; Pauline M Rudd; David M Lee Journal: Arthritis Rheum Date: 2010-08
Authors: B Cylwik; A Chludzinska; L Chrostek; E Gruszewska; E Gindzienska-Sieskiewicz; M Szmitkowski; S Sierakowski Journal: Z Rheumatol Date: 2012-04 Impact factor: 1.372
Authors: Altan Ercan; Michael G Barnes; Melissa Hazen; Heather Tory; Lauren Henderson; Fatma Dedeoglu; Robert C Fuhlbrigge; Alexei Grom; Ingrid A Holm; Mark Kellogg; Susan Kim; Barbara Adamczyk; Pauline M Rudd; Mary Beth Son; Robert P Sundel; Dirk Foell; David N Glass; Susan D Thompson; Peter A Nigrovic Journal: Arthritis Rheum Date: 2012-09
Authors: J A Cremata; L Sorell; R Montesino; R Garcia; M Mata; G Cabrera; J A Galvan; G Garcia; R Valdes; J A Garrote Journal: Clin Exp Immunol Date: 2003-09 Impact factor: 4.330
Authors: M Holland; K Takada; T Okumoto; N Takahashi; K Kato; D Adu; A Ben-Smith; L Harper; C O S Savage; R Jefferis Journal: Clin Exp Immunol Date: 2002-07 Impact factor: 4.330