OBJECTIVE: To examine the comparative efficacies of Arsenicum Album 30C and 200C and three administrative modes in protecting against the genotoxic effects produced by Arsenic trioxide injection in mice. DESIGN: Healthy mice, Mus musculus, were intraperitoneally injected with a 0.004% solution of As2O3 @1 ml/100 gms of body weight. Genotoxic effects were assessed through chromosome aberrations (CA), micronucleated erythrocytes (MNE), mitotic index (MI) and sperm head anomaly (SHA) studies, keeping suitable succussed alcohol fed (positive) and As2O3 untreated normal (negative) controls. The As2O3 treated mice were divided into three subgroups, which were orally administered with the drug a) prior to, b) after and c) both prior to and after injection of As2O3 at specific fixation intervals. RESULTS: While the CA, MNE and SHA were reduced in the drug fed series as compared to respective controls, the MI showed an apparent increase. The combined pre- and post-feeding of Arsenicum album was found to be most effective in reducing the genotoxic effects of As2O3 i200C was more effective than 30C. CONCLUSION: Arsenicum Album reduces the genotoxic effect of arsenic poisoning.
OBJECTIVE: To examine the comparative efficacies of Arsenicum Album 30C and 200C and three administrative modes in protecting against the genotoxic effects produced by Arsenic trioxide injection in mice. DESIGN: Healthy mice, Mus musculus, were intraperitoneally injected with a 0.004% solution of As2O3 @1 ml/100 gms of body weight. Genotoxic effects were assessed through chromosome aberrations (CA), micronucleated erythrocytes (MNE), mitotic index (MI) and sperm head anomaly (SHA) studies, keeping suitable succussed alcohol fed (positive) and As2O3 untreated normal (negative) controls. The As2O3 treated mice were divided into three subgroups, which were orally administered with the drug a) prior to, b) after and c) both prior to and after injection of As2O3 at specific fixation intervals. RESULTS: While the CA, MNE and SHA were reduced in the drug fed series as compared to respective controls, the MI showed an apparent increase. The combined pre- and post-feeding of Arsenicum album was found to be most effective in reducing the genotoxic effects of As2O3 i200C was more effective than 30C. CONCLUSION: Arsenicum Album reduces the genotoxic effect of arsenic poisoning.