OBJECTIVE AND DESIGN: To investigate whether mast cells (MCs) and chymase, the major protease of murine MCs, were involved in a chronic fibroproliferative disorder of the paws associated with type II collagen (CII)-induced arthritis. MATERIALS: Eighteen DBA/1J mice were divided into 3 groups and were used to study fibroproliferative changes in paws elicited by immunization. TREATMENT: Arthritis was induced by immunization with CII, which was intradermally injected as an emulsion made with adjuvant. A booster shot was done 3 weeks after the initial shot. A group with no treatment and that received adjuvant alone served as control. METHODS: Twelve weeks after the booster shot, inflammation of the paws was evaluated for pathological and biochemical indices. Chymase activity was determined with a chromogenic peptide substrate. RESULTS: In CII-immunized group, collagen bundles accumulated around the destructed joints. In accordance with the pathological findings, MC density in the affected paws was increased (154.8+/-13.3/mm2; p<0.05 vs. control) and chymase activity was also increased (29.5+/-2.8 mU/mg protein; p<0.01 vs. control). CONCLUSIONS: The present results demonstrate increases in MCs and chymase in fibroproliferative paws of collagen-induced arthritic mice.
OBJECTIVE AND DESIGN: To investigate whether mast cells (MCs) and chymase, the major protease of murine MCs, were involved in a chronic fibroproliferative disorder of the paws associated with type II collagen (CII)-induced arthritis. MATERIALS: Eighteen DBA/1J mice were divided into 3 groups and were used to study fibroproliferative changes in paws elicited by immunization. TREATMENT: Arthritis was induced by immunization with CII, which was intradermally injected as an emulsion made with adjuvant. A booster shot was done 3 weeks after the initial shot. A group with no treatment and that received adjuvant alone served as control. METHODS: Twelve weeks after the booster shot, inflammation of the paws was evaluated for pathological and biochemical indices. Chymase activity was determined with a chromogenic peptide substrate. RESULTS: In CII-immunized group, collagen bundles accumulated around the destructed joints. In accordance with the pathological findings, MC density in the affected paws was increased (154.8+/-13.3/mm2; p<0.05 vs. control) and chymase activity was also increased (29.5+/-2.8 mU/mg protein; p<0.01 vs. control). CONCLUSIONS: The present results demonstrate increases in MCs and chymase in fibroproliferative paws of collagen-induced arthritic mice.
Authors: Ricardo T Paniagua; Orr Sharpe; Peggy P Ho; Steven M Chan; Anna Chang; John P Higgins; Beren H Tomooka; Fiona M Thomas; Jason J Song; Stuart B Goodman; David M Lee; Mark C Genovese; Paul J Utz; Lawrence Steinman; William H Robinson Journal: J Clin Invest Date: 2006-09-14 Impact factor: 14.808
Authors: Ryohei F Tsuji; Marian Szczepanik; Ivana Kawikova; Vipin Paliwal; Regis A Campos; Atsuko Itakura; Moe Akahira-Azuma; Nicole Baumgarth; Leonore A Herzenberg; Philip W Askenase Journal: J Exp Med Date: 2002-11-18 Impact factor: 14.307
Authors: Jeffery M Cowden; Fuqu Yu; Homayon Banie; Mandana Farahani; Ping Ling; Steven Nguyen; Jason P Riley; Mai Zhang; Jian Zhu; Paul J Dunford; Robin L Thurmond Journal: Ann Rheum Dis Date: 2013-10-14 Impact factor: 19.103