| Literature DB >> 10433483 |
K Fukuda1, Y Hibiya, M Mutoh, M Koshiji, S Akao, H Fujiwara.
Abstract
The enzyme cyclooxygenase-2 (COX-2) is abundantly expressed in colon cancer cells and plays a key role in colon tumorigenesis. Compounds inhibiting COX-2 transcriptional activity have therefore potentially a chemopreventive property against colon tumor formation. An assay method for estimating COX-2 transcriptional activity in human colon cancer cells was established using a beta-galactosidase reporter gene system, and examination was made of various medicinal herbs and their ingredients for an inhibitory effect on COX-2 transcriptional activity. We found that berberine, an isoquinoline alkaloid present in plants of the genera Berberis and Coptis, effectively inhibits COX-2 transcriptional activity in colon cancer cells in a dose- and time-dependent manner at concentrations higher than 0.3 microM. The present findings may further explain the mechanism of anti-inflammatory and anti-tumor promoting effects of berberine.Entities:
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Year: 1999 PMID： 10433483 DOI： 10.1016/s0378-8741(98)00162-7
Source DB: PubMed Journal: J Ethnopharmacol ISSN： 0378-8741 Impact factor: 4.360