Literature DB >> 10432746

Hydrolysis of some mRNA 5'-cap analogs catalyzed by the human Fhit protein--and lupin ApppA hydrolases.

E Bojarska1, R Kraciuk, J Wierzchowski, Z Wieczorek, J Stepiński, M Jankowska, E Starzyńska, A Guranowski, E Darzynkiewicz.   

Abstract

Hydrolysis of the following four cap analogs: m7G(5')ppp(5')A, m7G(5')ppp(5')m6A, m7G(5')ppp(5')m2'OG and m7G(5')ppp(5')2'dG catalyzed by homogeneous human Fhit protein and yellow lupin Ap3A hydrolase has been investigated. The hydrolysis products were identified by HPLC analysis and the K(m) and Vmax values calculated based on the data obtained by the fluorimetric method.

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Year:  1999        PMID: 10432746     DOI: 10.1080/15257779908041666

Source DB:  PubMed          Journal:  Nucleosides Nucleotides        ISSN: 0732-8311


  1 in total

1.  Adenylylsulfate-ammonia adenylyltransferase activity is another inherent property of Fhit proteins.

Authors:  Anna M Wojdyła-Mamoń; Andrzej Guranowski
Journal:  Biosci Rep       Date:  2015-06-25       Impact factor: 3.840

  1 in total

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