Literature DB >> 10430034

A non-radioactive method for inexpensive quantitative RT-PCR.

M Maggiolini1, O Donzé, D Picard.   

Abstract

We present a novel method for quantitative RT-PCR that involves direct incorporation of digoxigenin-11-dUTP (DIG-dUTP) during amplification of cDNAs, separation of RT-PCR products by agarose gel electrophoresis, Southern transfer to a nylon membrane, and chemiluminescent detection with an anti-DIG antibody. The whole procedure can be done in about a day and has the following advantages: It is highly sensitive, specificity is confirmed by monitoring the size of the RT-PCR product, it is non-radioactive, quantitative, and does not require expensive specialized equipment.

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Year:  1999        PMID： 10430034     DOI： 10.1515/BC.1999.086

Source DB:  PubMed          Journal:  Biol Chem        ISSN： 1431-6730            Impact factor:   3.915


  10 in total

1.  Epidermal growth factor induces G protein-coupled receptor 30 expression in estrogen receptor-negative breast cancer cells.

Authors:  Lidia Albanito; Diego Sisci; Saveria Aquila; Elvira Brunelli; Adele Vivacqua; Antonio Madeo; Rosamaria Lappano; Deo Prakash Pandey; Didier Picard; Loredana Mauro; Sebastiano Andò; Marcello Maggiolini
Journal:  Endocrinology       Date:  2008-05-08       Impact factor: 4.736

2.  The food contaminants bisphenol A and 4-nonylphenol act as agonists for estrogen receptor alpha in MCF7 breast cancer cells.

Authors:  Adele Vivacqua; Anna Grazia Recchia; Giovanna Fasanella; Sabrina Gabriele; Amalia Carpino; Vittoria Rago; Maria Luisa Di Gioia; Antonella Leggio; Daniela Bonofiglio; Angelo Liguori; Marcello Maggiolini
Journal:  Endocrine       Date:  2003-12       Impact factor: 3.633

3.  Estrogenic GPR30 signalling induces proliferation and migration of breast cancer cells through CTGF.

Authors:  Deo Prakash Pandey; Rosamaria Lappano; Lidia Albanito; Antonio Madeo; Marcello Maggiolini; Didier Picard
Journal:  EMBO J       Date:  2009-01-15       Impact factor: 11.598

4.  G protein-coupled estrogen receptor mediates the up-regulation of fatty acid synthase induced by 17β-estradiol in cancer cells and cancer-associated fibroblasts.

Authors:  Maria Francesca Santolla; Rosamaria Lappano; Paola De Marco; Marco Pupo; Adele Vivacqua; Diego Sisci; Sergio Abonante; Domenico Iacopetta; Anna Rita Cappello; Vincenza Dolce; Marcello Maggiolini
Journal:  J Biol Chem       Date:  2012-11-07       Impact factor: 5.157

5.  Association of serum sex steroid receptor bioactivity and sex steroid hormones with breast cancer risk in postmenopausal women.

Authors:  Evangelia-Ourania Fourkala; Alexey Zaikin; Matthew Burnell; Aleksandra Gentry-Maharaj; Jeremy Ford; Richard Gunu; Christina Soromani; Guido Hasenbrink; Ian Jacobs; Anne Dawnay; Martin Widschwendter; Hella Lichtenberg-Fraté; Usha Menon
Journal:  Endocr Relat Cancer       Date:  2012-04-10       Impact factor: 5.678

6.  Effects of atrazine on estrogen receptor α- and G protein-coupled receptor 30-mediated signaling and proliferation in cancer cells and cancer-associated fibroblasts.

Authors:  Lidia Albanito; Rosamaria Lappano; Antonio Madeo; Adele Chimento; Eric R Prossnitz; Anna Rita Cappello; Vincenza Dolce; Sergio Abonante; Vincenzo Pezzi; Marcello Maggiolini
Journal:  Environ Health Perspect       Date:  2015-01-16       Impact factor: 9.031

7.  GPER mediates cardiotropic effects in spontaneously hypertensive rat hearts.

Authors:  Ernestina Marianna De Francesco; Tommaso Angelone; Teresa Pasqua; Marco Pupo; Maria Carmela Cerra; Marcello Maggiolini
Journal:  PLoS One       Date:  2013-08-09       Impact factor: 3.240

8.  G-protein-coupled receptor 30 and estrogen receptor-alpha are involved in the proliferative effects induced by atrazine in ovarian cancer cells.

Authors:  Lidia Albanito; Rosamaria Lappano; Antonio Madeo; Adele Chimento; Eric R Prossnitz; Anna Rita Cappello; Vincenza Dolce; Sergio Abonante; Vincenzo Pezzi; Marcello Maggiolini
Journal:  Environ Health Perspect       Date:  2008-07-22       Impact factor: 9.031

9.  HIF-1α/GPER signaling mediates the expression of VEGF induced by hypoxia in breast cancer associated fibroblasts (CAFs).

Authors:  Ernestina Marianna De Francesco; Rosamaria Lappano; Maria Francesca Santolla; Stefania Marsico; Arnaldo Caruso; Marcello Maggiolini
Journal:  Breast Cancer Res       Date:  2013       Impact factor: 6.466

10.  SIRT1 is involved in oncogenic signaling mediated by GPER in breast cancer.

Authors:  M F Santolla; S Avino; M Pellegrino; E M De Francesco; P De Marco; R Lappano; A Vivacqua; F Cirillo; D C Rigiracciolo; A Scarpelli; S Abonante; M Maggiolini
Journal:  Cell Death Dis       Date:  2015-07-30       Impact factor: 8.469
  10 in total

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