| Literature DB >> 10428481 |
S Nagafuchi1, H Katsuta, K Kogawa, T Akashi, S Kondo, Y Sakai, T Tsukiyama, D Kitamura, Y Niho, T Watanabe.
Abstract
A non-obese diabetic (NOD) mouse-derived embryonic stem (ES) cell line has been stably maintained in an undifferentiated state with a characteristic ES cell-like morphology, expressing the stem cell marker alkaline phosphatase, and displaying a normal diploid karyotype. After injecting the NOD-ES cells into blastocysts, chimeric mice were obtained. Small but significant numbers of lymphocytes expressed the NOD-derived MHC allele. When a chimeric mouse was mated with C57BL/6 mice, an agouti mouse was obtained, having the NOD-derived H-2 I-A(beta)g7 haplotype. Thus, an NOD-ES cell line which can differentiate into lymphocytes with potential for germ line transmission was successfully established.Entities:
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Year: 1999 PMID： 10428481 DOI： 10.1016/s0014-5793(99)00801-7
Source DB: PubMed Journal: FEBS Lett ISSN： 0014-5793 Impact factor: 4.124