| Literature DB >> 10427697 |
H Shibuya1, H Kobayashi, S Yoshida, S Kaneko, G G Park, I Kusakabe.
Abstract
The cDNAs coding for Mortierella vinacea alpha-galactosidases I and II were expressed in Saccharomyces cerevisiae under the control of the yeast GAL10 promoter. The recombinant enzymes purified to homogeneity from the culture filtrate were glycosylated, and had properties identical to those of the native enzymes except for improving the heat stability of alpha-galactosidase II and decreasing the specific activities of both enzymes.Entities:
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Year: 1999 PMID： 10427697 DOI： 10.1271/bbb.63.1096
Source DB: PubMed Journal: Biosci Biotechnol Biochem ISSN： 0916-8451 Impact factor: 2.043