Literature DB >> 10427074

The MTT [3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide] assay is a fast and reliable method for colorimetric determination of fungal cell densities.

F M Freimoser1, C A Jakob, M Aebi, U Tuor.   

Abstract

The entomopathogenic fungus Neozygites parvispora (Entomophthorales: Zygomycetes) grows in vitro as irregularly rod-shaped hyphal bodies in a complex medium. In order to simplify the medium composition and determine growth-promoting compounds for the cultivation of this fungus, we were looking for a rapid and quantitative method to estimate the number of living cells in small volumes of liquid culture. A colorimetric method for the determination of cell densities using MTT [3-(4,5-dimethylthiazol-2-yl)-2, 5-diphenyltetrazolium bromide] proved to be more accurate and timesaving than conventional hemocytometer counting.

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Year:  1999        PMID： 10427074      PMCID： PMC91559     

Source DB:  PubMed          Journal:  Appl Environ Microbiol        ISSN： 0099-2240            Impact factor:   4.792


  8 in total

1.  STUDIES ON SUCCINATE-TETRAZOLIUM REDUCTASE SYSTEMS. III. POINTS OF COUPLING OF FOUR DIFFERENT TETRAZOLIUM SALTS.

Authors:  T F SLATER; B SAWYER; U STRAEULI
Journal:  Biochim Biophys Acta       Date:  1963-11-08

2.  The MTT tetrazolium salt assay scrutinized: how to use this assay reliably to measure metabolic activity of cell cultures in vitro for the assessment of growth characteristics, IC50-values and cell survival.

Authors:  A M Sieuwerts; J G Klijn; H A Peters; J A Foekens
Journal:  Eur J Clin Chem Clin Biochem       Date:  1995-11

3.  Rapid colorimetric assay for cellular growth and survival: application to proliferation and cytotoxicity assays.

Authors:  T Mosmann
Journal:  J Immunol Methods       Date:  1983-12-16       Impact factor: 2.303

4.  Mechanism of cellular 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) reduction.

Authors:  Y Liu; D A Peterson; H Kimura; D Schubert
Journal:  J Neurochem       Date:  1997-08       Impact factor: 5.372

5.  Chemosensitivity testing of fresh human leukemia cells using both a dye exclusion assay and a tetrazolium dye (MTT) assay.

Authors:  D L Kirkpatrick; M Duke; T S Goh
Journal:  Leuk Res       Date:  1990       Impact factor: 3.156

Review 6.  Tetrazolium salts and formazans.

Authors:  F P Altman
Journal:  Prog Histochem Cytochem       Date:  1976

7.  An improved colorimetric assay for cell proliferation and viability utilizing the tetrazolium salt XTT.

Authors:  N W Roehm; G H Rodgers; S M Hatfield; A L Glasebrook
Journal:  J Immunol Methods       Date:  1991-09-13       Impact factor: 2.303

8.  Rapid colorimetric assay for cell growth and survival. Modifications to the tetrazolium dye procedure giving improved sensitivity and reliability.

Authors:  F Denizot; R Lang
Journal:  J Immunol Methods       Date:  1986-05-22       Impact factor: 2.303
  8 in total
  44 in total

1.  Chromogenic assay measuring opsonophagocytic killing capacities of antipneumococcal antisera.

Authors:  J S Lin; M K Park; M H Nahm
Journal:  Clin Diagn Lab Immunol       Date:  2001-05

2.  Functional assignment of YvgO, a novel set of purified and chemically characterized proteinaceous antifungal variants produced by Bacillus thuringiensis SF361.

Authors:  David C Manns; John J Churey; Randy W Worobo
Journal:  Appl Environ Microbiol       Date:  2012-02-03       Impact factor: 4.792

3.  A multiparametric organ toxicity predictor for drug discovery.

Authors:  Chirag N Patel; Sivakumar Prasanth Kumar; Rakesh M Rawal; Daxesh P Patel; Frank J Gonzalez; Himanshu A Pandya
Journal:  Toxicol Mech Methods       Date:  2019-10-29       Impact factor: 2.987

4.  H-gemcitabine: a new gemcitabine prodrug for treating cancer.

Authors:  Madhuri Dasari; Abhinav P Acharya; Dongin Kim; Seungjun Lee; Sungmun Lee; Jeanne Rhea; Ross Molinaro; Niren Murthy
Journal:  Bioconjug Chem       Date:  2012-12-26       Impact factor: 4.774

5.  Protective effects of curcumin on amyloid-β-induced neuronal oxidative damage.

Authors:  Han-Chang Huang; Ping Chang; Xue-Ling Dai; Zhao-Feng Jiang
Journal:  Neurochem Res       Date:  2012-04-04       Impact factor: 3.996

6.  Intercalative pyrimido[4',5':4,5]thieno(2,3-b)quinolines induce apoptosis in leukemic cells: a comparative study of methoxy and morpholino substitution.

Authors:  M S Shahabuddin; Mridula Nambiar; Gopal M Advirao; Sathees C Raghavan
Journal:  Invest New Drugs       Date:  2010-04-28       Impact factor: 3.850

7.  2-cinnamamido, 2-(3-phenylpropiolamido), and 2-(3-phenylpropanamido)benzamides: synthesis, antiproliferative activity, and mechanism of action.

Authors:  Demetrio Raffa; Benedetta Maggio; Maria Valeria Raimondi; Maria Grazia Cusimano; Giandomenico Amico; Anna Carollo; Pier Giulio Conaldi; Ruoli Bai; Ernest Hamel; Giuseppe Daidone
Journal:  Eur J Med Chem       Date:  2013-05-14       Impact factor: 6.514

8.  The influence of salinity on growth, morphology, leaf ultrastructure, and cell viability of the seagrass Halodule wrightii Ascherson.

Authors:  Chirle Ferreira; Carmen Simioni; Éder C Schmidt; Fernanda Ramlov; Marcelo Maraschin; Zenilda L Bouzon
Journal:  Protoplasma       Date:  2016-11-12       Impact factor: 3.356

9.  Knockdown of LMP1-induced miR-155 sensitizes nasopharyngeal carcinoma cells to radiotherapy in vitro.

Authors:  Yusheng Wang; L E Sun
Journal:  Oncol Lett       Date:  2016-03-31       Impact factor: 2.967

10.  Synthesis, antiproliferative activity and possible mechanism of action of novel 2-acetamidobenzamides bearing the 2-phenoxy functionality.

Authors:  Demetrio Raffa; Benedetta Maggio; Fabiana Plescia; Stella Cascioferro; Maria Valeria Raimondi; Gabriella Cancemi; Antonella D'Anneo; Marianna Lauricella; Maria Grazia Cusimano; Ruoli Bai; Ernest Hamel; Giuseppe Daidone
Journal:  Bioorg Med Chem       Date:  2015-08-28       Impact factor: 3.641
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