OBJECTIVE: To compare the angiotensinogen genotypes in normotensive and pre-eclamptic pregnancies in maternal and fetal samples. DESIGN: Prospective observational study. SETTING: University Hospital, Queen's Medical Centre, Nottingham. POPULATION: Forty-three women with pre-eclampsia and 84 normotensive pregnant women. Fetal samples were available for genotyping from 96% of the pregnancies. METHODS: Maternal and fetal DNA was genotyped at angiotensinogen codon 235 and at a dinucleotide repeat polymorphism in the 3' flanking region of the gene. Angiotensinogen and renin concentrations were measured in maternal plasma by radioimmunoassay. RESULTS: In contrast to earlier studies, no association was demonstrated between the angiotensinogen 235 Thr variant and pre-eclampsia. Normotensive pregnant women homozygous for this variant had significantly lower plasma angiotensinogen concentrations (median 2.2 ng AI/mL; IQR 1.8-3.0) than women homozygous for the 235 Met allele (3.6 ng AI/mL; IQR 2.5-4.1; P = 0.04). In pre-eclamptic pregnancies, 79% (11/14) of mothers heterozygous for the dinucleotide repeat allele designated A9 transmitted this allele to the fetus, more frequently than would be expected by chance (P = 0.02). The A9 allele was associated with low plasma angiotensinogen concentrations (P = 0.001) and high renin concentrations (P = 0.02) in normotensive women. CONCLUSIONS: There is no evidence that the angiotensinogen 235 Thr allele is associated with pre-eclampsia in the Nottingham population. The angiotensinogen 235 Thr allele is associated with low plasma angiotensinogen concentrations in normotensive pregnant women, in contrast to the high levels associated with this variant in non-pregnant women, suggesting that regulation of angiotensinogen expression in normal pregnancy may differ significantly from that in the non-pregnant state. There is preliminary evidence that maternal-fetal transmission of an angiotensinogen allele associated with low plasma angiotensinogen concentrations is associated with pre-eclampsia. Impaired generation of angiotensin II at the maternal-fetal interface may be a factor in the pathogenesis of pre-eclampsia.
OBJECTIVE: To compare the angiotensinogen genotypes in normotensive and pre-eclamptic pregnancies in maternal and fetal samples. DESIGN: Prospective observational study. SETTING: University Hospital, Queen's Medical Centre, Nottingham. POPULATION: Forty-three women with pre-eclampsia and 84 normotensive pregnant women. Fetal samples were available for genotyping from 96% of the pregnancies. METHODS: Maternal and fetal DNA was genotyped at angiotensinogen codon 235 and at a dinucleotide repeat polymorphism in the 3' flanking region of the gene. Angiotensinogen and renin concentrations were measured in maternal plasma by radioimmunoassay. RESULTS: In contrast to earlier studies, no association was demonstrated between the angiotensinogen 235 Thr variant and pre-eclampsia. Normotensive pregnant women homozygous for this variant had significantly lower plasma angiotensinogen concentrations (median 2.2 ng AI/mL; IQR 1.8-3.0) than women homozygous for the 235 Met allele (3.6 ng AI/mL; IQR 2.5-4.1; P = 0.04). In pre-eclamptic pregnancies, 79% (11/14) of mothers heterozygous for the dinucleotide repeat allele designated A9 transmitted this allele to the fetus, more frequently than would be expected by chance (P = 0.02). The A9 allele was associated with low plasma angiotensinogen concentrations (P = 0.001) and high renin concentrations (P = 0.02) in normotensive women. CONCLUSIONS: There is no evidence that the angiotensinogen 235 Thr allele is associated with pre-eclampsia in the Nottingham population. The angiotensinogen 235 Thr allele is associated with low plasma angiotensinogen concentrations in normotensive pregnant women, in contrast to the high levels associated with this variant in non-pregnant women, suggesting that regulation of angiotensinogen expression in normal pregnancy may differ significantly from that in the non-pregnant state. There is preliminary evidence that maternal-fetal transmission of an angiotensinogen allele associated with low plasma angiotensinogen concentrations is associated with pre-eclampsia. Impaired generation of angiotensin II at the maternal-fetal interface may be a factor in the pathogenesis of pre-eclampsia.
Authors: Valgerdur Steinthorsdottir; Ralph McGinnis; Nicholas O Williams; Lilja Stefansdottir; Gudmar Thorleifsson; Scott Shooter; João Fadista; Jon K Sigurdsson; Kirsi M Auro; Galina Berezina; Maria-Carolina Borges; Suzannah Bumpstead; Jonas Bybjerg-Grauholm; Irina Colgiu; Vivien A Dolby; Frank Dudbridge; Stephanie M Engel; Christopher S Franklin; Michael L Frigge; Yr Frisbaek; Reynir T Geirsson; Frank Geller; Solveig Gretarsdottir; Daniel F Gudbjartsson; Quaker Harmon; David Michael Hougaard; Tatyana Hegay; Anna Helgadottir; Sigrun Hjartardottir; Tiina Jääskeläinen; Hrefna Johannsdottir; Ingileif Jonsdottir; Thorhildur Juliusdottir; Noor Kalsheker; Abdumadjit Kasimov; John P Kemp; Katja Kivinen; Kari Klungsøyr; Wai K Lee; Mads Melbye; Zosia Miedzybrodska; Ashley Moffett; Dilbar Najmutdinova; Firuza Nishanova; Thorunn Olafsdottir; Markus Perola; Fiona Broughton Pipkin; Lucilla Poston; Gordon Prescott; Saedis Saevarsdottir; Damilya Salimbayeva; Paula Juliet Scaife; Line Skotte; Eleonora Staines-Urias; Olafur A Stefansson; Karina Meden Sørensen; Liv Cecilie Vestrheim Thomsen; Vinicius Tragante; Lill Trogstad; Nigel A B Simpson; Tamara Aripova; Juan P Casas; Anna F Dominiczak; James J Walker; Unnur Thorsteinsdottir; Ann-Charlotte Iversen; Bjarke Feenstra; Deborah A Lawlor; Heather Allison Boyd; Per Magnus; Hannele Laivuori; Nodira Zakhidova; Gulnara Svyatova; Kari Stefansson; Linda Morgan Journal: Nat Commun Date: 2020-11-25 Impact factor: 14.919
Authors: Christina K H Yu; Juan P Casas; Makrina D Savvidou; Manpreet K Sahemey; Kypros H Nicolaides; Aroon D Hingorani Journal: BMC Pregnancy Childbirth Date: 2006-03-16 Impact factor: 3.007