BACKGROUND: No previous report has shown the relationship between Helicobacter pylori infection and a direct sequence analysis of p53 gene mutation in a biopsy sample of human gastric mucosa. METHODS: A total of 60 endoscopic biopsies samples (21 patients with H. pylori-positive gastritis and 9 patients with H. pylori-negative gastritis), including antral mucosa and corpus mucosa, were used in this study. Direct DNA sequencing of exons 5, 6, 7, and 8 of the p53 gene was performed by the dyedeoxy terminator method. RESULTS: Mutations in the p53 gene were identified in non-hot spot codons in exon 7 and 8 in 11 of 21 samples (52.4%) from H. pylori-positive gastritis patients. There was no mutation in H. pylori-negative gastritis patients. CONCLUSIONS: This finding shows that H. pylori infection can induce p53 point mutations and appears to be involved in the pathway leading to dysplasia or carcinoma.
BACKGROUND: No previous report has shown the relationship between Helicobacter pyloriinfection and a direct sequence analysis of p53 gene mutation in a biopsy sample of human gastric mucosa. METHODS: A total of 60 endoscopic biopsies samples (21 patients with H. pylori-positive gastritis and 9 patients with H. pylori-negative gastritis), including antral mucosa and corpus mucosa, were used in this study. Direct DNA sequencing of exons 5, 6, 7, and 8 of the p53 gene was performed by the dyedeoxy terminator method. RESULTS: Mutations in the p53 gene were identified in non-hot spot codons in exon 7 and 8 in 11 of 21 samples (52.4%) from H. pylori-positive gastritispatients. There was no mutation in H. pylori-negative gastritispatients. CONCLUSIONS: This finding shows that H. pyloriinfection can induce p53 point mutations and appears to be involved in the pathway leading to dysplasia or carcinoma.
Authors: G Sisson; J Y Jeong; A Goodwin; L Bryden; N Rossler; S Lim-Morrison; A Raudonikiene; D E Berg; P S Hoffman Journal: J Bacteriol Date: 2000-09 Impact factor: 3.490
Authors: Stefan Kubicka; Christiane Claas; Sven Staab; Florian Kühnel; Lars Zender; Christian Trautwein; Siegfried Wagner; Karl Lenhard Rudolph; Michael Manns Journal: Dig Dis Sci Date: 2002-01 Impact factor: 3.199
Authors: Jie Wang; David S Chi; George B Kalin; Christina Sosinski; Lou Ellen Miller; Izabela Burja; Eapen Thomas Journal: Dig Dis Sci Date: 2002-01 Impact factor: 3.199
Authors: Juan-Bosco Lopez-Saez; Victoria Gómez-Biondi; Germán Santamaría-Rodriguez; Margarita Dominguez-Villar; Antonio Amaya-Vidal; Antonio Lorenzo-Peñuelas; Avelino Senra-Varela Journal: J Exp Clin Cancer Res Date: 2010-06-04