| Literature DB >> 10422884 |
Y Frobert1, M C Nevers, S Amadesi, H Volland, P Brune, P Geppetti, J Grassi, C Créminon.
Abstract
Thirty mouse monoclonal antibodies (mAbs) directed against rat calcitonin gene-related peptide-alpha (CGRP-alpha) have been obtained. These mAbs are classified in 2 groups, one recognizing the peptide N-terminus and the other binding the C-terminus. A two-site immunometric assay was developed using mAb CGRP-83 as capture antibody, whereas mAb CGRP-72 acts as tracer, covalently labeled with enzyme acetylcholinesterase. This assay appeared sensitive (limit of detection: 2 pg/ml) and precise, allowing quantitative measurement of all human and murine CGRP isoforms. The assay was used to determine specific concentrations of CGRP in different rat, mice and guinea pig samples. The validity of the test was demonstrated by HPLC fractionation experiments.Entities:
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Year: 1999 PMID： 10422884 DOI： 10.1016/s0196-9781(98)00172-7
Source DB: PubMed Journal: Peptides ISSN： 0196-9781 Impact factor: 3.750