Literature DB >> 10421489

Novel protein targets for organophosphorus compounds.

P Richards1, M Johnson, D Ray, C Walker.   

Abstract

Inhibition of tritiated di-isopropyl phosphorofluoridate labelling by a range of organophopshorus compounds was used to screen for novel OP-reactive targets in rat-brain homogenates. Analysis of target proteins was conducted by SDS/PAGE and detection of tritiated proteins using a thin layer chromatography (TLC) linear analyser. Two major sites of 3H-DFP labelling were found with relative molecular masses of 30 and 85 kDa. Rates of reaction of these labelling sites with a range of OP compounds were compared to that of acetylcholinesterase. The 30 kDa band was found to be more sensitive to paraoxon, dichlorvos and diazoxon than acetylcholinesterase. The 85 kDa band was found to be more sensitive to dichlorvos and diazoxon than acetylcholinesterase. Neither labelling band reacted with chlorfenvinphos or demeton-s-methyl at significant rates.

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Year:  1999        PMID： 10421489     DOI： 10.1016/s0009-2797(99)00064-2

Source DB:  PubMed          Journal:  Chem Biol Interact        ISSN： 0009-2797            Impact factor:   5.192


  9 in total

Review 1.  Protein adducts as biomarkers of exposure to organophosphorus compounds.

Authors:  Judit Marsillach; Lucio G Costa; Clement E Furlong
Journal:  Toxicology       Date:  2012-12-20       Impact factor: 4.221

2.  Oxidative stress resulting from exposure of a human salivary gland cells to paraoxon: an in vitro model for organophosphate oral exposure.

Authors:  John M Prins; Chih-Kai Chao; Saskia M Jacobson; Charles M Thompson; Kathleen M George
Journal:  Toxicol In Vitro       Date:  2014-01-29       Impact factor: 3.500

3.  Biomarkers of sensitivity and exposure in Washington state pesticide handlers.

Authors:  Jonathan N Hofmann; Matthew C Keifer; Harvey Checkoway; Anneclaire J De Roos; Federico M Farin; Richard A Fenske; Rebecca J Richter; Gerald van Belle; Clement E Furlong
Journal:  Adv Exp Med Biol       Date:  2010       Impact factor: 2.622

4.  Paraoxon-induced protein expression changes to SH-SY5Y cells.

Authors:  John M Prins; Kathleen M George; Charles M Thompson
Journal:  Chem Res Toxicol       Date:  2010-10-08       Impact factor: 3.739

Review 5.  Immunotoxicity of organophosphorous pesticides.

Authors:  Tamara Galloway; Richard Handy
Journal:  Ecotoxicology       Date:  2003 Feb-Aug       Impact factor: 2.823

Review 6.  Mass spectrometric analyses of organophosphate insecticide oxon protein adducts.

Authors:  Charles M Thompson; John M Prins; Kathleen M George
Journal:  Environ Health Perspect       Date:  2010-01       Impact factor: 9.031

7.  Serum cholinesterase inhibition in relation to paraoxonase-1 (PON1) status among organophosphate-exposed agricultural pesticide handlers.

Authors:  Jonathan N Hofmann; Matthew C Keifer; Clement E Furlong; Anneclaire J De Roos; Federico M Farin; Richard A Fenske; Gerald van Belle; Harvey Checkoway
Journal:  Environ Health Perspect       Date:  2009-06-09       Impact factor: 9.031

8.  Protein binding of isofluorophate in vivo after coexposure to multiple chemicals.

Authors:  John S Vogel; Garrett A Keating; Bruce A Buchholz
Journal:  Environ Health Perspect       Date:  2002-12       Impact factor: 9.031

9.  Efficacy of antidotes (midazolam, atropine and HI-6) on nerve agent induced molecular and neuropathological changes.

Authors:  Golime RamaRao; Prachiti Afley; Jyothiranjan Acharya; Bijoy Krishna Bhattacharya
Journal:  BMC Neurosci       Date:  2014-04-04       Impact factor: 3.288
  9 in total

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