Literature DB >> 10419975

Molecular cloning, sequence analysis, and characterization of a penicillin-resistant DD-carboxypeptidase of Myxococcus xanthus.

Y Kimura1, Y Takashima, Y Tokumasu, M Sato.   

Abstract

We have cloned a gene, pdcA, from the genomic library of Myxococcus xanthus with an oligonucleotide probe representing conserved regions of penicillin-resistant DD-carboxypeptidases. The amino- and carboxy-terminal halves of the predicted pdcA gene product showed significant sequence similarity to N-acetylmuramoyl-L-alanine amidase and penicillin-resistant DD-carboxypeptidase, respectively. The pdcA gene was expressed in Escherichia coli, and the characteristics of the gene product were similar to those of DD-carboxypeptidase (VanY) of vancomycin-resistant enterococci. No apparent changes in cell growth, sporulation, or germination were observed in pdcA deletion mutants.

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Year:  1999        PMID： 10419975      PMCID： PMC103608     

Source DB:  PubMed          Journal:  J Bacteriol        ISSN： 0021-9193            Impact factor:   3.490


  40 in total

1.  Synergism between morphogenetic mutants of Myxococcus xanthus.

Authors:  D C Hagen; A P Bretscher; D Kaiser
Journal:  Dev Biol       Date:  1978-06       Impact factor: 3.582

2.  Mutational evidence for identity of penicillin-binding protein 5 in Escherichia coli with the major D-alanine carboxypeptidase IA activity.

Authors:  M Matsuhashi; S Tamaki; S J Curtis; J L Strominger
Journal:  J Bacteriol       Date:  1979-01       Impact factor: 3.490

Review 3.  The chemical basis for the action of the vancomycin group of antibiotics.

Authors:  H R Perkins; M Nieto
Journal:  Ann N Y Acad Sci       Date:  1974-05-10       Impact factor: 5.691

4.  Isolation of bacteriophage MX4, a generalized transducing phage for Myxococcus xanthus.

Authors:  J M Campos; J Geisselsoder; D R Zusman
Journal:  J Mol Biol       Date:  1978-02-25       Impact factor: 5.469

5.  Changes in penicillin-binding proteins during sporulation of Bacillus subtilis.

Authors:  M O Sowell; C E Buchanan
Journal:  J Bacteriol       Date:  1983-03       Impact factor: 3.490

6.  Mode of action of Myxococcus xanthus antibiotic TA.

Authors:  D Zafriri; E Rosenberg; D Mirelman
Journal:  Antimicrob Agents Chemother       Date:  1981-02       Impact factor: 5.191

7.  Spherical E. coli due to elevated levels of D-alanine carboxypeptidase.

Authors:  Z Markiewicz; J K Broome-Smith; U Schwarz; B G Spratt
Journal:  Nature       Date:  1982-06-24       Impact factor: 49.962

8.  The exocellular DD-carboxypeptidase of Streptomyces albus G: a metallo (Zn2+) enzyme.

Authors:  O Dideberg; B Joris; J M Frere; J M Ghuysen; G Weber; R Robaye; J M Delbrouck; I Roelandts
Journal:  FEBS Lett       Date:  1980-08-11       Impact factor: 4.124

9.  Bactericidal action of an antibiotic produced by Myxococcus xanthus.

Authors:  E Rosenberg; B Vaks; A Zuckerberg
Journal:  Antimicrob Agents Chemother       Date:  1973-11       Impact factor: 5.191

10.  Deletion of the penicillin-binding protein 6 gene of Escherichia coli.

Authors:  J K Broome-Smith; B G Spratt
Journal:  J Bacteriol       Date:  1982-11       Impact factor: 3.490
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  2 in total

1.  Genomic and molecular analysis of phage CMP1 from Clavibacter michiganensis subspecies michiganensis.

Authors:  Johannes Wittmann; Karl-Heinz Gartemann; Rudolf Eichenlaub; Brigitte Dreiseikelmann
Journal:  Bacteriophage       Date:  2011-01

2.  Identification of the full set of Listeria monocytogenes penicillin-binding proteins and characterization of PBPD2 (Lmo2812).

Authors:  Dorota Korsak; Zdzislaw Markiewicz; Gabriel O Gutkind; Juan A Ayala
Journal:  BMC Microbiol       Date:  2010-09-15       Impact factor: 3.605
  2 in total

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