| Literature DB >> 10417717 |
J Le Gourrierec1, Y F Li, D X Zhou.
Abstract
GT-1 belongs to the class of trihelix DNA-binding proteins and binds to a promoter sequence found in many different genes. Data presented in this report show that GT-1 contains a trans-activation function in yeast and in plant cells. However, in tobacco BY-2 protoplasts, this activity functions only when an internal region containing the DNA-binding domain is deleted. Gel-shift and co-immunoprecipitation assays have revealed that GT-1 can interact with and stabilize the TFIIA-TBP-TATA complex. These results suggest that GT-1 may activate transcription through direct inter- action with the transcriptional pre-initiation complex.Entities:
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Year: 1999 PMID： 10417717 DOI： 10.1046/j.1365-313x.1999.00482.x
Source DB: PubMed Journal: Plant J ISSN： 0960-7412 Impact factor: 6.417