Literature DB >> 10417315

Adenylosuccinate synthase from Saccharomyces cerevisiae: homologous overexpression, purification and characterization of the recombinant protein.

G Lipps1, G Krauss.   

Abstract

Adenylosuccinate synthase (EC 6.3.4.4) catalyses the first committed step in the synthesis of adenosine. We have overexpressed the cloned gene of Saccharomyces cerevisiae (ADE12) in S. cerevisiae. The recombinant enzyme exhibits similar kinetic behaviour to that of the native enzyme purified from S. cerevisiae. This ter-reactant dimeric enzyme shows Michaelis-Menten kinetics only with IMP. l-Aspartate and GTP display a weak negative co-operativity (Hill coefficient 0. 8-0.9). This negative co-operativity has not yet been reported for adenylosuccinate synthases from other organisms. Another unusual feature of the enzyme from S. cerevisiae is its negligible inhibition by adenine nucleotides and its pronounced inhibition by Cl(-) ions.

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Year:  1999        PMID： 10417315      PMCID： PMC1220389     

Source DB:  PubMed          Journal:  Biochem J        ISSN： 0264-6021            Impact factor:   3.857


  34 in total

1.  Purification, crystallization, and properties of adenylosuccinate synthetase from rat skeletal muscle.

Authors:  H Ogawa; H Shiraki; Y Matsuda; K Kakiuchi; H Nakagawa
Journal:  J Biochem       Date:  1977-04       Impact factor: 3.387

2.  Adenylosuccinate synthetase from Azotobacter vinelandii: purification, properties and steady-state kinetics.

Authors:  G D Markham; G H Reed
Journal:  Arch Biochem Biophys       Date:  1977-11       Impact factor: 4.013

3.  A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding.

Authors:  M M Bradford
Journal:  Anal Biochem       Date:  1976-05-07       Impact factor: 3.365

4.  Cooperativity in associating proteins. Monomer-dimer equilibrium coupled to ligand binding.

Authors:  A Levitzki; J Schlessinger
Journal:  Biochemistry       Date:  1974-12-03       Impact factor: 3.162

5.  Spectrophotometric determination of protein concentration in cell extracts containing tRNA's and rRNA's.

Authors:  B Ehresmann; P Imbault; J H Weil
Journal:  Anal Biochem       Date:  1973-08       Impact factor: 3.365

6.  Purification of adenylosuccinate synthetase from rabbit skeletal muscle.

Authors:  K M Muirhead; S H Bishop
Journal:  J Biol Chem       Date:  1974-01-25       Impact factor: 5.157

7.  Regulation of the biosynthesis of purine nucleotides in Schizosaccharomyces pombe. 3. Kinetic studies of adenylosuccinate synthetase.

Authors:  M Nagy; M Djembo-Taty
Journal:  Biochim Biophys Acta       Date:  1973-05-05

8.  Initial rate studies of adenylosuccinate synthetase with product and competitive inhibitors.

Authors:  F B Rudolph; H J Fromm
Journal:  J Biol Chem       Date:  1969-07-25       Impact factor: 5.157

9.  Adenylosuccinate synthetase in rat liver: the existence of two types and their regulatory roles.

Authors:  Y Matsuda; H Ogawa; S Fukutome; H Shiraki; H Nakagawa
Journal:  Biochem Biophys Res Commun       Date:  1977-09-23       Impact factor: 3.575

10.  Relationship of glycolytic intermediates, glycolytic enzymes, and ammonia to glycogen metabolism during sporulation in the yeast Saccharomyces cerevisiae.

Authors:  W A Fonzi; M Shanley; D J Opheim
Journal:  J Bacteriol       Date:  1979-01       Impact factor: 3.490
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  9 in total

1.  An empirical extremum principle for the hill coefficient in ligand-protein interactions showing negative cooperativity.

Authors:  Hagai Abeliovich
Journal:  Biophys J       Date:  2005-04-15       Impact factor: 4.033

2.  Overexpression, purification, crystallization and preliminary crystallographic studies of a hyperthermophilic adenylosuccinate synthetase from Pyrococcus horikoshii OT3.

Authors:  Xiaoying Wang; Ryogo Akasaka; Chie Takemoto; Satoshi Morita; Machiko Yamaguchi; Takaho Terada; Mikako Shirozu; Shigeyuki Yokoyama; Shilin Chen; Shuyi Si; Yong Xie
Journal:  Acta Crystallogr Sect F Struct Biol Cryst Commun       Date:  2011-11-25

3.  Molecular cloning and characterization of a novel muscle adenylosuccinate synthetase, AdSSL1, from human bone marrow stromal cells.

Authors:  Hongying Sun; Nan Li; Xiaojian Wang; Taoyong Chen; Liyun Shi; Lihuang Zhang; Jianli Wang; Tao Wan; Xuetao Cao
Journal:  Mol Cell Biochem       Date:  2005-01       Impact factor: 3.396

4.  Electron Microscopy Pathology of ADSSL1 Myopathy.

Authors:  Hyung Jun Park; Jee Eun Lee; Gyeong Seon Choi; Heasoo Koo; Soo Jeong Han; Jeong Hyun Yoo; Young Chul Choi; Kee Duk Park
Journal:  J Clin Neurol       Date:  2016-11-17       Impact factor: 3.077

5.  Analysis of transcriptional profiles of Saccharomyces cerevisiae exposed to bisphenol A.

Authors:  Ceyhun Bereketoglu; Kazim Yalcin Arga; Serpil Eraslan; Bulent Mertoglu
Journal:  Curr Genet       Date:  2016-07-26       Impact factor: 3.886

6.  Panorama of the distal myopathies.

Authors:  Marco Savarese; Jaakko Sarparanta; Anna Vihola; Per Harald Jonson; Mridul Johari; Salla Rusanen; Peter Hackman; Bjarne Udd
Journal:  Acta Myol       Date:  2020-12-01

7.  Adenylsuccinate Synthetase MoADE12 Plays Important Roles in the Development and Pathogenicity of the Rice Blast Fungus.

Authors:  Zhen Zhang; Zhongna Hao; Rongyao Chai; Haiping Qiu; Yanli Wang; Jiaoyu Wang; Guochang Sun
Journal:  J Fungi (Basel)       Date:  2022-07-26

8.  Antibacterial Activity and Components of the Methanol-Phase Extract from Rhizomes of Pharmacophagous Plant Alpinia officinarum Hance.

Authors:  Junfeng Fu; Yaping Wang; Meng Sun; Yingwei Xu; Lanming Chen
Journal:  Molecules       Date:  2022-07-05       Impact factor: 4.927

9.  Omics-based analyses revealed metabolic responses of Clostridium acetobutylicum to lignocellulose-derived inhibitors furfural, formic acid and phenol stress for butanol fermentation.

Authors:  Huanhuan Liu; Jing Zhang; Jian Yuan; Xiaolong Jiang; Lingyan Jiang; Guang Zhao; Di Huang; Bin Liu
Journal:  Biotechnol Biofuels       Date:  2019-04-27       Impact factor: 6.040
  9 in total

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