| Literature DB >> 10416678 |
H E Valentin1, D L Broyles, L A Casagrande, S M Colburn, W L Creely, P A DeLaquil, H M Felton, K A Gonzalez, K L Houmiel, K Lutke, D A Mahadeo, T A Mitsky, S R Padgette, S E Reiser, S Slater, D M Stark, R T Stock, D A Stone, N B Taylor, G M Thorne, M Tran, K J Gruys.
Abstract
The genes encoding the polyhydroxyalkanoate (PHA) biosynthetic pathway in Ralstonia eutropha (3-ketothiolase, phaA or bktB; acetoacetyl-CoA reductase, phaB; and PHA synthase, phaC) were engineered for plant plastid targeting and expressed using leaf (e35S) or seed-specific (7s or lesquerella hydroxylase) promoters in Arabidopsis and Brassica. PHA yields in homozygous transformants were 12-13% of the dry mass in homozygous Arabidopsis plants and approximately 7% of the seed weight in seeds from heterozygous canola plants. When a threonine deaminase was expressed in addition to bktB, phaB and phaC, a copolyester of 3-hydroxybutyrate and 3-hydroxyvalerate was produced in both Arabidopsis and Brassica.Entities:
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Year: 1999 PMID： 10416678 DOI： 10.1016/s0141-8130(99)00045-8
Source DB: PubMed Journal: Int J Biol Macromol ISSN： 0141-8130 Impact factor: 6.953