OBJECTIVE: For a detailed understanding of the complex traits growth and fat storage, a dissection into single genetic entities is mandatory. Therefore, blood plasma concentrations of hormones and the expression of selected genes were measured in extremely differentiated mouse lines. Genes were selected as candidates which might influence the complex traits body weight and fat accumulation, and which are located in chromosomal regions recently identified to affect trait differences between the lines. SUBJECTS AND MEASUREMENTS: The mouse lines were selected for high body weight (DU6), high carcass protein content (DU6P) and unselected controls (DUKs). In the selected lines DU6 and DU6P, mean body weights at the age of six weeks were about twice as high as the DUKs, whereas total fat weight was increased 2.2-fold in DU6 mice compared to DU6P and 3.2-fold in comparison to DUKs. Blood plasma concentrations of insulin-like growth factor 1 (IGF-1), growth hormone (GH), insulin and leptin, were measured in all lines at three weeks and at six weeks of age. Expression patterns of the genes encoding growth hormone (Gh), insulin-like growth factor 1 (Igf1), lipoprotein lipase (Lpl), glycerolphosphate dehydrogenase 1 (GDC-1), and adipocyte protein 2 (Ap2) were analyzed by Northern blot hybridization. RESULTS: In line DU6, highly significant increased concentrations of insulin and leptin were observed at six weeks of age; at this stage, IGF-1 concentrations were elevated in the two selected lines compared to controls with maximal concentrations of IGF-1 and GH in DU6P. The amount of mRNA for GH in the pituitary gland, for Igf1 in the liver and for LPL in epididymal fat tissue was significantly elevated in the two selected lines compared to controls at the age of three weeks, but not at six weeks. IGF-1 and GDC-1 mRNA concentrations were significantly higher in the DU6 mice than in the DU6P (P < 0.01) and the DUKs (P < 0.001) mice examined at both ages. CONCLUSIONS: The results prove line-specific concentrations of the analyzed hormones and the transcription amounts of Gh, Igf1, GDC-1 and Lpl. The measured differences are either direct genetic effects or secondary changes, resulting from different food consumption.
OBJECTIVE: For a detailed understanding of the complex traits growth and fat storage, a dissection into single genetic entities is mandatory. Therefore, blood plasma concentrations of hormones and the expression of selected genes were measured in extremely differentiated mouse lines. Genes were selected as candidates which might influence the complex traits body weight and fat accumulation, and which are located in chromosomal regions recently identified to affect trait differences between the lines. SUBJECTS AND MEASUREMENTS: The mouse lines were selected for high body weight (DU6), high carcass protein content (DU6P) and unselected controls (DUKs). In the selected lines DU6 and DU6P, mean body weights at the age of six weeks were about twice as high as the DUKs, whereas total fat weight was increased 2.2-fold in DU6 mice compared to DU6P and 3.2-fold in comparison to DUKs. Blood plasma concentrations of insulin-like growth factor 1 (IGF-1), growth hormone (GH), insulin and leptin, were measured in all lines at three weeks and at six weeks of age. Expression patterns of the genes encoding growth hormone (Gh), insulin-like growth factor 1 (Igf1), lipoprotein lipase (Lpl), glycerolphosphate dehydrogenase 1 (GDC-1), and adipocyte protein 2 (Ap2) were analyzed by Northern blot hybridization. RESULTS: In line DU6, highly significant increased concentrations of insulin and leptin were observed at six weeks of age; at this stage, IGF-1 concentrations were elevated in the two selected lines compared to controls with maximal concentrations of IGF-1 and GH in DU6P. The amount of mRNA for GH in the pituitary gland, for Igf1 in the liver and for LPL in epididymal fat tissue was significantly elevated in the two selected lines compared to controls at the age of three weeks, but not at six weeks. IGF-1 and GDC-1 mRNA concentrations were significantly higher in the DU6 mice than in the DU6P (P < 0.01) and the DUKs (P < 0.001) mice examined at both ages. CONCLUSIONS: The results prove line-specific concentrations of the analyzed hormones and the transcription amounts of Gh, Igf1, GDC-1 and Lpl. The measured differences are either direct genetic effects or secondary changes, resulting from different food consumption.
Authors: Thomas Walther; Nils Dietrich; Martina Langhammer; Marzena Kucia; Harald Hammon; Ulla Renne; Wolf-Eberhard Siems; Cornelia C Metges Journal: PLoS One Date: 2011-03-09 Impact factor: 3.240
Authors: Michael Walz; Luong Chau; Christina Walz; Mandy Sawitzky; Daniela Ohde; Julia Brenmoehl; Armin Tuchscherer; Martina Langhammer; Friedrich Metzger; Christine Höflich; Andreas Hoeflich Journal: Cells Date: 2020-06-22 Impact factor: 6.600
Authors: Annika Müller-Eigner; Adrián Sanz-Moreno; Irene de-Diego; Anuroop Venkateswaran Venkatasubramani; Martina Langhammer; Raffaele Gerlini; Birgit Rathkolb; Antonio Aguilar-Pimentel; Tanja Klein-Rodewald; Julia Calzada-Wack; Lore Becker; Sergio Palma-Vera; Benedikt Gille; Ignasi Forne; Axel Imhof; Chen Meng; Christina Ludwig; Franziska Koch; John T Heiker; Angela Kuhla; Vanessa Caton; Julia Brenmoehl; Henry Reyer; Jennifer Schoen; Helmut Fuchs; Valerie Gailus-Durner; Andreas Hoeflich; Martin Hrabe de Angelis; Shahaf Peleg Journal: Commun Biol Date: 2022-05-03
Authors: Ulla Renne; Martina Langhammer; Julia Brenmoehl; Christina Walz; Anja Zeissler; Armin Tuchscherer; Marion Piechotta; Rudolf J Wiesner; Maximilian Bielohuby; Andreas Hoeflich Journal: PLoS One Date: 2013-11-13 Impact factor: 3.240