Literature DB >> 10406132

Leginon: a system for fully automated acquisition of 1000 electron micrographs a day.

C S Potter1, H Chu, B Frey, C Green, N Kisseberth, T J Madden, K L Miller, K Nahrstedt, J Pulokas, A Reilein, D Tcheng, D Weber, B Carragher.   

Abstract

We have developed a system to automatically acquire large numbers of acceptable quality images from specimens of negatively stained catalase, a biological protein which forms crystals. In this paper we will describe the details of the system architecture and analyze the performance of the system as compared to a human operator. The ultimate goal of the system if to automate the process of acquiring cryo-electron micrographs.

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Year:  1999        PMID： 10406132     DOI： 10.1016/s0304-3991(99)00043-1

Source DB:  PubMed          Journal:  Ultramicroscopy        ISSN： 0304-3991            Impact factor:   2.689


  66 in total

Review 1.  The mysterious RAMP proteins and their roles in small RNA-based immunity.

Authors:  Ruiying Wang; Hong Li
Journal:  Protein Sci       Date:  2012-04       Impact factor: 6.725

2.  An automated pipeline to screen membrane protein 2D crystallization.

Authors:  Changki Kim; Martin Vink; Minghui Hu; James Love; David L Stokes; Iban Ubarretxena-Belandia
Journal:  J Struct Funct Genomics       Date:  2010-03-27

3.  Detection of membrane protein two-dimensional crystals in living cells.

Authors:  E J Gualtieri; F Guo; D J Kissick; J Jose; R J Kuhn; W Jiang; G J Simpson
Journal:  Biophys J       Date:  2011-01-05       Impact factor: 4.033

Review 4.  Precise beam-tilt alignment and collimation are required to minimize the phase error associated with coma in high-resolution cryo-EM.

Authors:  Robert M Glaeser; Dieter Typke; Peter C Tiemeijer; James Pulokas; Anchi Cheng
Journal:  J Struct Biol       Date:  2010-12-21       Impact factor: 2.867

Review 5.  Generalized single-particle cryo-EM--a historical perspective.

Authors:  Joachim Frank
Journal:  Microscopy (Oxf)       Date:  2015-11-12       Impact factor: 1.571

Review 6.  Automated data collection in single particle electron microscopy.

Authors:  Yong Zi Tan; Anchi Cheng; Clinton S Potter; Bridget Carragher
Journal:  Microscopy (Oxf)       Date:  2015-12-15       Impact factor: 1.571

7.  Mechanism of human antibody-mediated neutralization of Marburg virus.

Authors:  Andrew I Flyak; Philipp A Ilinykh; Charles D Murin; Tania Garron; Xiaoli Shen; Marnie L Fusco; Takao Hashiguchi; Zachary A Bornholdt; James C Slaughter; Gopal Sapparapu; Curtis Klages; Thomas G Ksiazek; Andrew B Ward; Erica Ollmann Saphire; Alexander Bukreyev; James E Crowe
Journal:  Cell       Date:  2015-02-26       Impact factor: 41.582

8.  Automated 100-position specimen loader and image acquisition system for transmission electron microscopy.

Authors:  Jonathan Lefman; Robert Morrison; Sriram Subramaniam
Journal:  J Struct Biol       Date:  2006-12-16       Impact factor: 2.867

9.  Modular software platform for low-dose electron microscopy and tomography.

Authors:  Michael P Marsh; Juan T Chang; Christopher R Booth; Nathan L Liang; Michael F Schmid; Wah Chiu
Journal:  J Microsc       Date:  2007-12       Impact factor: 1.758

10.  Towards automated screening of two-dimensional crystals.

Authors:  Anchi Cheng; Albert Leung; Denis Fellmann; Joel Quispe; Christian Suloway; James Pulokas; Priyanka D Abeyrathne; Joseph S Lam; Bridget Carragher; Clinton S Potter
Journal:  J Struct Biol       Date:  2007-09-25       Impact factor: 2.867
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