Literature DB >> 10405431

Molecular detection and identification of intimin alleles in pathogenic Escherichia coli by multiplex PCR.

S D Reid1, D J Betting, T S Whittam.   

Abstract

A multiplex PCR was designed to detect the eae gene and simultaneously identify specific alleles in pathogenic Escherichia coli. The method was tested on 87 strains representing the diarrheagenic E. coli clones. The results show that the PCR assay accurately detects eae and resolves alleles encoding the alpha, beta, and gamma intimin variants.

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Year:  1999        PMID： 10405431      PMCID： PMC85327     

Source DB:  PubMed          Journal:  J Clin Microbiol        ISSN： 0095-1137            Impact factor:   5.948


  9 in total

Review 1.  The epidemiology of infections caused by Escherichia coli O157:H7, other enterohemorrhagic E. coli, and the associated hemolytic uremic syndrome.

Authors:  P M Griffin; R V Tauxe
Journal:  Epidemiol Rev       Date:  1991       Impact factor: 6.222

2.  The complete sequence of the locus of enterocyte effacement (LEE) from enteropathogenic Escherichia coli E2348/69.

Authors:  S J Elliott; L A Wainwright; T K McDaniel; K G Jarvis; Y K Deng; L C Lai; B P McNamara; M S Donnenberg; J B Kaper
Journal:  Mol Microbiol       Date:  1998-04       Impact factor: 3.501

3.  Molecular typing of enterohemorrhagic Escherichia coli O157:H7 isolates in Japan by using pulsed-field gel electrophoresis.

Authors:  H Izumiya; J Terajima; A Wada; Y Inagaki; K I Itoh; K Tamura; H Watanabe
Journal:  J Clin Microbiol       Date:  1997-07       Impact factor: 5.948

4.  Molecular evolution and mosaic structure of alpha, beta, and gamma intimins of pathogenic Escherichia coli.

Authors:  E A McGraw; J Li; R K Selander; T S Whittam
Journal:  Mol Biol Evol       Date:  1999-01       Impact factor: 16.240

Review 5.  Diarrheagenic Escherichia coli.

Authors:  J P Nataro; J B Kaper
Journal:  Clin Microbiol Rev       Date:  1998-01       Impact factor: 26.132

Review 6.  Escherichia coli serotype O157:H7: novel vehicles of infection and emergence of phenotypic variants.

Authors:  P Feng
Journal:  Emerg Infect Dis       Date:  1995 Apr-Jun       Impact factor: 6.883

7.  Clonal relationships among Escherichia coli strains that cause hemorrhagic colitis and infantile diarrhea.

Authors:  T S Whittam; M L Wolfe; I K Wachsmuth; F Orskov; I Orskov; R A Wilson
Journal:  Infect Immun       Date:  1993-05       Impact factor: 3.441

8.  Insertion site of the locus of enterocyte effacement in enteropathogenic and enterohemorrhagic Escherichia coli differs in relation to the clonal phylogeny of the strains.

Authors:  L H Wieler; T K McDaniel; T S Whittam; J B Kaper
Journal:  FEMS Microbiol Lett       Date:  1997-11-01       Impact factor: 2.742

9.  Detection of intimins alpha, beta, gamma, and delta, four intimin derivatives expressed by attaching and effacing microbial pathogens.

Authors:  J Adu-Bobie; G Frankel; C Bain; A G Goncalves; L R Trabulsi; G Douce; S Knutton; G Dougan
Journal:  J Clin Microbiol       Date:  1998-03       Impact factor: 5.948
  9 in total
  31 in total

1.  Produce isolates of the Escherichia coli Ont:H52 serotype that carry both Shiga toxin 1 and stable toxin genes.

Authors:  Steven R Monday; Christina Keys; Patricia Hanson; Yuelian Shen; Thomas S Whittam; Peter Feng
Journal:  Appl Environ Microbiol       Date:  2006-04       Impact factor: 4.792

2.  Intimin types determined by heteroduplex mobility assay of intimin gene (eae)-positive Escherichia coli strains.

Authors:  Kenitiro Ito; Mariko Iida; Mitsugu Yamazaki; Kazuo Moriya; Sanae Moroishi; Jun Yatsuyanagi; Takayuki Kurazono; Noriaki Hiruta; Orn-Anong Ratchtrachenchai
Journal:  J Clin Microbiol       Date:  2007-01-17       Impact factor: 5.948

3.  Molecular evolution of the intimin gene in O111 clones of pathogenic Escherichia coli.

Authors:  Cheryl L Tarr; Thomas S Whittam
Journal:  J Bacteriol       Date:  2002-01       Impact factor: 3.490

4.  EHEC Adhesins.

Authors:  Brian D McWilliams; Alfredo G Torres
Journal:  Microbiol Spectr       Date:  2014

5.  Complete nucleotide sequence and analysis of the locus of enterocyte Effacement from rabbit diarrheagenic Escherichia coli RDEC-1.

Authors:  C Zhu; T S Agin; S J Elliott; L A Johnson; T E Thate; J B Kaper; E C Boedeker
Journal:  Infect Immun       Date:  2001-04       Impact factor: 3.441

6.  Representational difference analysis between Afa/Dr diffusely adhering Escherichia coli and nonpathogenic E. coli K-12.

Authors:  Anne-Beatrice Blanc-Potard; Colin Tinsley; Isabel Scaletsky; Chantal Le Bouguenec; Julie Guignot; Alain L Servin; Xavier Nassif; Marie-Francoise Bernet-Camard
Journal:  Infect Immun       Date:  2002-10       Impact factor: 3.441

7.  Genes related to long polar fimbriae of pathogenic Escherichia coli strains as reliable markers to identify virulent isolates.

Authors:  Alfredo G Torres; Miguel Blanco; Patricio Valenzuela; Terry M Slater; Shilpa D Patel; Ghizlane Dahbi; Cecilia López; Ximena Fernández Barriga; Jesús E Blanco; Tânia A T Gomes; Roberto Vidal; Jorge Blanco
Journal:  J Clin Microbiol       Date:  2009-06-03       Impact factor: 5.948

8.  Escherichia coli isolates that carry vat, fyuA, chuA, and yfcV efficiently colonize the urinary tract.

Authors:  Rachel R Spurbeck; Paul C Dinh; Seth T Walk; Ann E Stapleton; Thomas M Hooton; Lisa K Nolan; Kwang Sik Kim; James R Johnson; Harry L T Mobley
Journal:  Infect Immun       Date:  2012-09-10       Impact factor: 3.441

9.  Blinded application of microscopy, bacteriological culture, immunoassays and PCR to detect gastrointestinal pathogens from faecal samples of patients with community-acquired diarrhoea.

Authors:  C F L Amar; C East; E Maclure; J McLauchlin; C Jenkins; P Duncanson; D R A Wareing
Journal:  Eur J Clin Microbiol Infect Dis       Date:  2004-06-16       Impact factor: 3.267

10.  Genomic diversity of pathogenic Escherichia coli of the EHEC 2 clonal complex.

Authors:  Galeb S Abu-Ali; David W Lacher; Lukas M Wick; Weihong Qi; Thomas S Whittam
Journal:  BMC Genomics       Date:  2009-07-03       Impact factor: 3.969
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