| Literature DB >> 10403044 |
M T Peracchia1, S Harnisch, H Pinto-Alphandary, A Gulik, J C Dedieu, D Desmaële, J d'Angelo, R H Müller, P Couvreur.
Abstract
The in vitro protein-rejecting properties of PEG-coated polyalkylcyanoacrylate (PACA) nanoparticles were for the first time visualized after freeze-fracture of the nanoparticles pre-incubated with fibrinogen as a model blood protein. The reduced protein association to the nanoparticles was evidenced also by two-dimensional PAGE after incubation of the nanoparticles with human plasma. In vivo experiments showed the 'stealth' long-circulating properties of the PEGylated nanoparticles after intravenous administration to mice. Thus, the images obtained after nanoparticle-protein incubation were predictive of the behavior observed in vivo. In conclusion, freeze-fracture analysis represents a novel and original qualitative approach to investigate the interactions between proteins and particulate systems.Entities:
Mesh：
Substances：
Year: 1999 PMID： 10403044 DOI： 10.1016/s0142-9612(99)00021-6
Source DB: PubMed Journal: Biomaterials ISSN： 0142-9612 Impact factor: 12.479