Literature DB >> 10402038

Urine as another potential source for template DNA in polymerase chain reaction (PCR).

S Hayakawa1, O Takenaka.   

Abstract

This technical note examines the potential for preparing template DNA in polymerase chain reactions (PCR) from urine in Japanese macaques (Macaca fuscata). Microsatellite band patterns from urine samples showed close agreement with those of blood and fecal samples, and only a few hundred microl of urine yielded a template DNA for PCR. This research will increase the opportunity for scientists to examine the genetic backgrounds of their target animals by using non-invasive sample collection in the wild.

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Year:  1999        PMID: 10402038     DOI: 10.1002/(SICI)1098-2345(1999)48:4<299::AID-AJP5>3.0.CO;2-G

Source DB:  PubMed          Journal:  Am J Primatol        ISSN: 0275-2565            Impact factor:   2.371


  5 in total

1.  Wild chimpanzee infant urine and saliva sampled noninvasively usable for DNA analyses.

Authors:  Eiji Inoue; Miho Inoue-Murayama; Osamu Takenaka; Toshisada Nishida
Journal:  Primates       Date:  2006-11-17       Impact factor: 2.163

2.  Urine as a high-quality source of host genomic DNA from wild populations.

Authors:  Andrew T Ozga; Timothy H Webster; Ian C Gilby; Melissa A Wilson; Rebecca S Nockerts; Michael L Wilson; Anne E Pusey; Yingying Li; Beatrice H Hahn; Anne C Stone
Journal:  Mol Ecol Resour       Date:  2020-10-17       Impact factor: 7.090

Review 3.  Strategies for determining kinship in wild populations using genetic data.

Authors:  Veronika Städele; Linda Vigilant
Journal:  Ecol Evol       Date:  2016-07-29       Impact factor: 2.912

4.  How Behavior of Nontarget Species Affects Perceived Accuracy of Scat Detection Dog Surveys.

Authors:  Karen E DeMatteo; Linsey W Blake; Julie K Young; Barbara Davenport
Journal:  Sci Rep       Date:  2018-09-14       Impact factor: 4.379

Review 5.  Using genetics to understand the dynamics of wild primate populations.

Authors:  Linda Vigilant; Katerina Guschanski
Journal:  Primates       Date:  2009-01-28       Impact factor: 2.163

  5 in total

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