Literature DB >> 10397854

Pilot scale production and isolation of recombinant NAD+- and NADP+-specific formate dehydrogenases.

V I Tishkov1, A G Galkin, V V Fedorchuk, P A Savitsky, A M Rojkova, H Gieren, M R Kula.   

Abstract

The expression of the recombinant wild-type NAD+- and mutant NADP+-dependent formate dehydrogenases (EC 1.2.1.2., FDH) from the methanol-utilizing bacterium Pseudomonas sp. 101 in Escherichia coli cells has been improved to produce active and soluble enzyme up to the level of 50% of total soluble proteins. The cultivation process for E. coli/pFDH8a and E. coli/pFDH8aNP cells was optimized and scaled up to a volume of 100 L. A downstream purification process has been developed to produce technical grade NAD+- and NADP+-specific formate dehydrogenases in pilot scale, utilizing extraction in aqueous two-phase systems. Copyright 1999 John Wiley & Sons, Inc.

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Year:  1999        PMID： 10397854

Source DB:  PubMed          Journal:  Biotechnol Bioeng        ISSN： 0006-3592            Impact factor:   4.530


  9 in total

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Authors:  Katja Schirwitz; Andrea Schmidt; Victor S Lamzin
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8.  Engineering Cofactor Specificity of a Thermostable Phosphite Dehydrogenase for a Highly Efficient and Robust NADPH Regeneration System.

Authors:  Gamal Nasser Abdel-Hady; Takeshi Ikeda; Takenori Ishida; Hisakage Funabashi; Akio Kuroda; Ryuichi Hirota
Journal:  Front Bioeng Biotechnol       Date:  2021-04-01

9.  Genetic fusion of P450 BM3 and formate dehydrogenase towards self-sufficient biocatalysts with enhanced activity.

Authors:  Arsenij Kokorin; Pavel D Parshin; Patrick J Bakkes; Anastasia A Pometun; Vladimir I Tishkov; Vlada B Urlacher
Journal:  Sci Rep       Date:  2021-11-04       Impact factor: 4.379
  9 in total

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