S Bandak1, A Ramu, Y Barenholz, A Gabizon. 1. Sharet Institute of Oncology, Hadassah Hebrew University Medical Center, Jerusalem, Israel. suzan@md2.huji.ac.il
Abstract
PURPOSE: The aim of this study was to investigate the stability of doxorubicin encapsulated in polyethyleneglycol-coated liposomes (Doxil) under UV-A light. METHODS: High performance liquid chromatography and a fluorimetric method were used to quantify doxorubicin in bulk solution and doxorubicin in Doxil formulation. RESULTS: The photodegradation of Doxil was significantly lower in comparison to the photodegradation of the free drug and showed no concentration dependency at the measured concentration range of 5-50 microg/ml. During and after UV-A irradiation, there was no leakage of the drug from liposomes to the medium. After induced leakage of doxorubicin from the liposomes by the ionophore nigericin, the degradation kinetics of Doxil were identical to that of free doxorubicin. CONCLUSIONS: High intraliposomal doxorubicin concentration and intraliposomal acidic pH are the two critical factors that protect DXR in Doxil from UV-A degradation.
PURPOSE: The aim of this study was to investigate the stability of doxorubicin encapsulated in polyethyleneglycol-coated liposomes (Doxil) under UV-A light. METHODS: High performance liquid chromatography and a fluorimetric method were used to quantify doxorubicin in bulk solution and doxorubicin in Doxil formulation. RESULTS: The photodegradation of Doxil was significantly lower in comparison to the photodegradation of the free drug and showed no concentration dependency at the measured concentration range of 5-50 microg/ml. During and after UV-A irradiation, there was no leakage of the drug from liposomes to the medium. After induced leakage of doxorubicin from the liposomes by the ionophore nigericin, the degradation kinetics of Doxil were identical to that of free doxorubicin. CONCLUSIONS: High intraliposomal doxorubicin concentration and intraliposomal acidic pH are the two critical factors that protect DXR in Doxil from UV-A degradation.
Authors: A Gabizon; R Catane; B Uziely; B Kaufman; T Safra; R Cohen; F Martin; A Huang; Y Barenholz Journal: Cancer Res Date: 1994-02-15 Impact factor: 12.701
Authors: Katya Kamenova; Lyubomira Radeva; Krassimira Yoncheva; Filip Ublekov; Martin A Ravutsov; Maya K Marinova; Svilen P Simeonov; Aleksander Forys; Barbara Trzebicka; Petar D Petrov Journal: Polymers (Basel) Date: 2022-09-05 Impact factor: 4.967