Induction of the rat Cu/Zn superoxide dismutase gene through the peroxisome proliferator-responsive element by arachidonic acid.

The Cu/Zn superoxide dismutase (SOD1) catalyzes the dismutation of superoxide radicals produced from biological oxidation and environmental stresses. From the sequence analysis of transcription factor binding sites, the peroxisome proliferator-responsive element (PPRE) was located between nt -797 and -786 of the 5'-flanking sequence of the SOD1 gene. A promoter region was fused to a chloramphenicol acetyl-transferase gene, and the resultant construct was transiently transfected into HepG2 cells. The expression of the SOD1 gene was induced by arachidonic acid (AA). Functional analyses of the PPRE site by deletion, mutations, and the heterologous promoter system confirmed the induction of the SOD1 gene by AA through the PPRE site. Gel mobility shift assays showed AA inducible binding of the peroxisome proliferator-activated receptor (PPAR) to the PPRE. The intensity of PPAR binding was also increased by the treatment of retinoic acid (RA) and 9-cis retinoic acid (9-cis RA). These results suggest that the PPRE participates in the induction of the rat SOD1 gene by the peroxisome proliferator.