BACKGROUND: Polymerase chain reaction assays of the peripheral blood mononuclear cells (PBMC) and plasma may facilitate the diagnosis of neonatal herpes simplex virus (HSV). METHODS: Assays for HSV DNA were submitted from at least 1 specimen site (PBMC, plasma or cerebrospinal fluid) in 11 consecutive cases of neonatal HSV infection. RESULTS: HSV DNA was detected by PCR in the PBMC of 6 of 10 infants tested (60%), the plasma of 4 of 6 tested (67%) and the cerebrospinal fluid of 4 of 11 tested (36%). CONCLUSIONS: HSV viremia is more frequent than previously appreciated, and detection of HSV DNA in PBMC and plasma is a useful diagnostic tool, particularly in infants without skin lesions.
BACKGROUND: Polymerase chain reaction assays of the peripheral blood mononuclear cells (PBMC) and plasma may facilitate the diagnosis of neonatal herpes simplex virus (HSV). METHODS: Assays for HSV DNA were submitted from at least 1 specimen site (PBMC, plasma or cerebrospinal fluid) in 11 consecutive cases of neonatal HSV infection. RESULTS: HSV DNA was detected by PCR in the PBMC of 6 of 10 infants tested (60%), the plasma of 4 of 6 tested (67%) and the cerebrospinal fluid of 4 of 11 tested (36%). CONCLUSIONS:HSV viremia is more frequent than previously appreciated, and detection of HSV DNA in PBMC and plasma is a useful diagnostic tool, particularly in infants without skin lesions.
Authors: Javier S Burgos; Carlos Ramirez; Fernando Guzman-Sanchez; Juan M Alfaro; Isabel Sastre; Fernando Valdivieso Journal: J Virol Date: 2006-03 Impact factor: 5.103
Authors: Sarah J Kopp; Andrew H Karaba; Laura K Cohen; Ghazal Banisadr; Richard J Miller; William J Muller Journal: J Virol Date: 2012-10-24 Impact factor: 5.103
Authors: Ann J Melvin; Kathleen M Mohan; Joshua T Schiffer; Linda M Drolette; Amalia Magaret; Lawrence Corey; Anna Wald Journal: J Pediatr Date: 2014-12-06 Impact factor: 4.406
Authors: Andrew H Karaba; Laura K Cohen; Taly Glaubach; Sarah J Kopp; Jennifer L Reichek; Hawke H Yoon; Xiaotian T Zheng; William J Muller Journal: J Pediatric Infect Dis Soc Date: 2012-05-03 Impact factor: 3.164