Literature DB >> 10388725

Specific detection of the gene for the extracellular neutral protease of Bacillus cereus by PCR and blot hybridization.

H Bach1, D Errampalli, K T Leung, H Lee, A Hartmann, J T Trevors, J C Munch.   

Abstract

A pair of primers and a gene probe for the amplification and detection of the Bacillus cereus neutral protease gene (NPRC) were developed. Specificity for the npr genes of the B. cereus group members B. cereus, B. mycoides, and B. thuringiensis was shown. Restriction polymorphism patterns of the PCR products confirmed the presence of the NPRC gene in all three species.

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Year:  1999        PMID： 10388725      PMCID： PMC91478     

Source DB:  PubMed          Journal:  Appl Environ Microbiol        ISSN： 0099-2240            Impact factor:   4.792


  9 in total

1.  The role of the pro-sequence in the processing and secretion of the thermolysin-like neutral protease from Bacillus cereus.

Authors:  D R Wetmore; S L Wong; R S Roche
Journal:  Mol Microbiol       Date:  1992-06       Impact factor: 3.501

2.  Combination of 16S rRNA-targeted oligonucleotide probes with flow cytometry for analyzing mixed microbial populations.

Authors:  R I Amann; B J Binder; R J Olson; S W Chisholm; R Devereux; D A Stahl
Journal:  Appl Environ Microbiol       Date:  1990-06       Impact factor: 4.792

3.  The primary structure of Bacillus cereus neutral proteinase and comparison with thermolysin and Bacillus subtilis neutral proteinase.

Authors:  W Sidler; E Niederer; F Suter; H Zuber
Journal:  Biol Chem Hoppe Seyler       Date:  1986-07

4.  Cloning of the nprA gene for neutral protease A of Bacillus thuringiensis and effect of in vivo deletion of nprA on insecticidal crystal protein.

Authors:  W P Donovan; Y Tan; A C Slaney
Journal:  Appl Environ Microbiol       Date:  1997-06       Impact factor: 4.792

5.  DNA competition studies within the Bacillus cereus group of bacilli.

Authors:  H J Somerville; M L Jones
Journal:  J Gen Microbiol       Date:  1972-11

6.  Comparative analysis of Bacillus anthracis, Bacillus cereus, and related species on the basis of reverse transcriptase sequencing of 16S rRNA.

Authors:  C Ash; J A Farrow; M Dorsch; E Stackebrandt; M D Collins
Journal:  Int J Syst Bacteriol       Date:  1991-07

7.  Distribution and identification of proteolytic Bacillus spp. in paddy field soil under rice cultivation.

Authors:  K Watanabe; K Hayano
Journal:  Can J Microbiol       Date:  1993-07       Impact factor: 2.419

8.  Characterization of Bacillus thuringiensis and related bacteria by ribosomal RNA gene restriction fragment length polymorphisms.

Authors:  F G Priest; D A Kaji; Y B Rosato; V P Canhos
Journal:  Microbiology       Date:  1994-05       Impact factor: 2.777

9.  Comparative analysis of the 16S to 23S ribosomal intergenic spacer sequences of Bacillus thuringiensis strains and subspecies and of closely related species.

Authors:  S N Bourque; J R Valero; M C Lavoie; R C Levesque
Journal:  Appl Environ Microbiol       Date:  1995-04       Impact factor: 4.792
  9 in total
  1 in total

1.  Detection of Bacillus cereus group bacteria from cardboard and paper with real-time PCR.

Authors:  Outi Priha; Katri Hallamaa; Maria Saarela; Laura Raaska
Journal:  J Ind Microbiol Biotechnol       Date:  2004-04-03       Impact factor: 3.346
  1 in total

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