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Literature DB >> 10388271

Axenic cultivation and characterization of Giardia lamblia isolated from humans in Korea.

S J Park¹, T S Yong, H W Yang, D H Lee, K Lee.

Abstract

Inoculating of human fecal cysts to suckling Mongolian gerbils, two Giardia lamblia isolates, K1 and K2, were established as axenic cultures. Using this in vitro culture, both isolates were characterized as a "medium-rate grower" upon its growth pattern. These two Giardia isolates were grouped by using two genetic analysis. With genetic analysis of SSU-rDNA sequences, both K1 and K2 were found as members of Hopkins' group 1, despite some nucleotide differences noticed in K2 (5 differences/292 bases). The other genetic study used PCR-RFLP of the tim (triose phosphate isomerase) gene. Both of K1 and K2 were found to belong to Nash's group 2. Our results suggest that Nash's group 2 can not be a separate group, but a part of Hopkins' group 1.

Entities: Disease Species

Mesh：

Year: 1999 PMID： 10388271 PMCID： PMC2733057 DOI： 10.3347/kjp.1999.37.2.121

Source DB: PubMed Journal: Korean J Parasitol ISSN： 0023-4001 Impact factor: 1.341

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