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Literature DB >> 103868

Immunohistochemical demonstration of carbonic anhydrase.

Abstract

Rabbits were immunized using human erythroxyte carbonic anhydrase B (HCA B) purified by the modified methods of Armstrong et al. (1966) and Bernstein and Schraer (1972). The globulin fraction was isolated by ammonium sulphate precipitation. The anti-HCA B globulin was specific, when judged using the double diffusion technique of Ouchterlony and immunoelectrophoresis. No cross reaction with human erythrocyte carbonic anhydrase C was found, but cross reactions with erythrocyte carbonic anhydrase from rat, mouse and guinea pig were observed. Flurorescein isothiocyanate conjugated goat anti-rabbit globulin was used for the localization of HCA B in tissue sections and erythrocytes on slides.

Entities: Chemical Gene Species

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Year: 1978 PMID： 103868 DOI： 10.1007/bf00495718

Source DB: PubMed Journal: Histochemistry ISSN： 0301-5564

28 in total

1. Localization of carbonic anhydrase in the nervous system.

Authors: E GIACOBINI
Journal: Science Date: 1961-11-10 Impact factor: 47.728

2. Carbonic anhydrase in the cornea.

Authors: G Lönnerholm
Journal: Acta Physiol Scand Date: 1974-01

3. Large-scale preparation of the human carbonic anhydrases.

Authors: L E Henderson; D Henriksson
Journal: Anal Biochem Date: 1973-01 Impact factor: 3.365

Review 4. Carbonic anhydrase: isoenzymes, properties, distribution, and functional significance.

Authors: M J Carter
Journal: Biol Rev Camb Philos Soc Date: 1972-11

5. Genetic and phylogenetic variation in the different molecular forms of mammalian erythrocyte carbonic anhydrases.

Authors: R E Tashian; D C Shreffler; T B Shows
Journal: Ann N Y Acad Sci Date: 1968-06-14 Impact factor: 5.691

6. Separation of human tissue alkaline phosphatases by electrophoresis on acrylamide disc gels.

Authors: I Smith; P J Lightstone; J D Perry
Journal: Clin Chim Acta Date: 1968-03 Impact factor: 3.786

7. Localization of carbonic anhydrase in avian gastric mucosa, shell gland and bone by immunohistochemistry.

Authors: C V Gay; E J Faleski; H Schraer; R Schraer
Journal: J Histochem Cytochem Date: 1974-08 Impact factor: 2.479

8. A critical evaluation of the histochemical methods for carbonic anhydrase.

Authors: T F Muther
Journal: J Histochem Cytochem Date: 1972-05 Impact factor: 2.479

9. Purification and properties of an avian carbonic anhydrase from the erythrocytes of Gallus domesticus.

Authors: R S Bernstein; R Schraer
Journal: J Biol Chem Date: 1972-02-25 Impact factor: 5.157

10. Serologic comparisons of the carbonic anhydrases of primate erythrocytes.

Authors: L Nonno; H Herschman; L Levine
Journal: Arch Biochem Biophys Date: 1970-02 Impact factor: 4.013

15 in total

1. Immunohistochemical localization of human carbonic anhydrase isoenzyme C.

Authors: T Kumpulainen
Journal: Histochemistry Date: 1979-08

2. Carbonic anhydrase activity in peripheral T-lymphocytes and appearance of the activity during their maturation in the thymus. A histochemical demonstration.

Authors: S Parkkila; H Rajaniemi
Journal: Histochemistry Date: 1989

10. Immunohistochemical localization of carbonic anhydrase I and II in eccrine sweat glands from control subjects and patients with cystic fibrosis.

Authors: J V Briggman; R E Tashian; S S Spicer
Journal: Am J Pathol Date: 1983-09 Impact factor: 4.307

Immunohistochemical demonstration of carbonic anhydrase.

1. Localization of carbonic anhydrase in the nervous system.

2. Carbonic anhydrase in the cornea.

3. Large-scale preparation of the human carbonic anhydrases.

Review 4. Carbonic anhydrase: isoenzymes, properties, distribution, and functional significance.

5. Genetic and phylogenetic variation in the different molecular forms of mammalian erythrocyte carbonic anhydrases.

6. Separation of human tissue alkaline phosphatases by electrophoresis on acrylamide disc gels.

7. Localization of carbonic anhydrase in avian gastric mucosa, shell gland and bone by immunohistochemistry.

8. A critical evaluation of the histochemical methods for carbonic anhydrase.

9. Purification and properties of an avian carbonic anhydrase from the erythrocytes of Gallus domesticus.

10. Serologic comparisons of the carbonic anhydrases of primate erythrocytes.

1. Immunohistochemical localization of human carbonic anhydrase isoenzyme C.

2. Carbonic anhydrase activity in peripheral T-lymphocytes and appearance of the activity during their maturation in the thymus. A histochemical demonstration.

3. Immunocytochemical localization of carbonic anhydrase on ultrathin frozen sections with protein A-gold.

4. Immunohistochemistry of carbonic anhydrase in human placenta and fetal membranes.

5. Immunohistochemical demonstration of carbonic anhydrase isoenzyme C in the epithelium of the human ciliary processes.

6. Immunohistochemical detection of bovine ruminal carbonic anhydrase isoenzyme.

7. Immunohistochemical demonstration of extracellular carbonic anhydrase in epiphyseal growth cartilage.

8. Carbonic anhydrase isoenzymes II and I are present in the zona glomerulosa cells of the human adrenal gland.

9. High active isoenzyme of carbonic anhydrase in rat calvaria osteoclasts. Immunohistochemical study.

10. Immunohistochemical localization of carbonic anhydrase I and II in eccrine sweat glands from control subjects and patients with cystic fibrosis.