Literature DB >> 10386623

In vivo synthesis of complex N-glycans by expression of human N-acetylglucosaminyltransferase I in the filamentous fungus Trichoderma reesei.

M Maras1, A De Bruyn, W Vervecken, J Uusitalo, M Penttilä, R Busson, P Herdewijn, R Contreras.   

Abstract

The human N-acetylglucosaminyltransferase I gene was introduced in the genome of Trichoderma reesei strain VTT-D-80133. Expression was studied after induction from the cellobiohydrolase I promoter. Successful in vivo transfer of GlcNAc was demonstrated by analyzing the neutral N-glycans which were synthesized on cellobiohydrolase I. Final proof of the formation of GlcNAcMan5GlcNAc2 was obtained by NMR analysis.

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Year:  1999        PMID： 10386623     DOI： 10.1016/s0014-5793(99)00648-1

Source DB:  PubMed          Journal:  FEBS Lett        ISSN： 0014-5793            Impact factor:   4.124


  4 in total

1.  Recombinant Protein Production and Purification of Insoluble Proteins.

Authors:  Neus Ferrer-Miralles; Paolo Saccardo; José Luis Corchero; Elena Garcia-Fruitós
Journal:  Methods Mol Biol       Date:  2022

2.  Paracoccin from Paracoccidioides brasiliensis; purification through affinity with chitin and identification of N-acetyl-beta-D-glucosaminidase activity.

Authors:  Fausto Bruno dos Reis Almeida; Leandro Licursi de Oliveira; Marcelo Valle de Sousa; Maria Cristina Roque Barreira; Ebert Seixas Hanna
Journal:  Yeast       Date:  2010-02       Impact factor: 3.239

3.  N-glycan modification in Aspergillus species.

Authors:  Elke Kainz; Andreas Gallmetzer; Christian Hatzl; Juergen H Nett; Huijuan Li; Thorsten Schinko; Robert Pachlinger; Harald Berger; Yazmid Reyes-Dominguez; Andreas Bernreiter; Tillmann Gerngross; Stefan Wildt; Joseph Strauss
Journal:  Appl Environ Microbiol       Date:  2007-12-14       Impact factor: 4.792

4.  Potential role of N-acetyl glucosamine in Aspergillus fumigatus-assisted Chlorella pyrenoidosa harvesting.

Authors:  Arghya Bhattacharya; Megha Mathur; Pushpendar Kumar; Anushree Malik
Journal:  Biotechnol Biofuels       Date:  2019-07-10       Impact factor: 6.040
  4 in total

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