| Literature DB >> 10385972 |
B Henry1, H Desvaux, M Pristchepa, P Berthault, Y M Zhang, J M Mallet, J Esnault, P Sinaÿ.
Abstract
The structure and conformation of the synthetic pentasaccharide Gal(beta 1-4){Fuc(alpha 1-3)}GlcNAc(beta 1-3)Gal(beta 1-4)Glc-beta OMe of the Lewis(X) family has been determined by NMR spectroscopy in dimethyl sulfoxide and methanol. In these solvents, the binding constants with calcium have been evaluated as 9.5 and 29.6 M-1, respectively. Study of the interaction sites has been achieved through the use of paramagnetic divalent cations and distance triangulation methods. Two regions have been found, the first one in the vicinity of the fucose unit, the second one closer to the lactose part.Entities:
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Year: 1999 PMID： 10385972 DOI： 10.1016/s0008-6215(98)00301-2
Source DB: PubMed Journal: Carbohydr Res ISSN： 0008-6215 Impact factor: 2.104